Heat shock response in hyperthermophilic microorganisms
... and J Baross, unpublished results]. The densest protein band produced in both organisms during heat-shock, as seen by pulse labeling with 35S-labeled amino acids, had a mass of approximately 60 kDa and was shown to be a chaperonin [see below]. In ES4, other proteins of various masses were also produ ...
... and J Baross, unpublished results]. The densest protein band produced in both organisms during heat-shock, as seen by pulse labeling with 35S-labeled amino acids, had a mass of approximately 60 kDa and was shown to be a chaperonin [see below]. In ES4, other proteins of various masses were also produ ...
... the siderophore ferrioxamine (Llamas et al., 2006). Siderophores are high-affinity ironchelating compounds that are produced and secreted by bacteria to solubilize the minute amounts of bioavailable iron present in the environment (Ratledge and Dover, 2000; Wandersman and Delepelaire, 2004). P. aeru ...
Creating Multiple Sequence Alignments
... A1. Start MEGA4 by using Start\Programs\BioInformatics\MEGA4. A2. In the MEGA4 window, go to Alignment|Alignment Explorer/CLUSTAL. Select ‘Create a new alignment’, and click on OK. Click on [NO] for protein sequence alignment. A3. Sequences can be entered either from FASTA files or by hand. We will ...
... A1. Start MEGA4 by using Start\Programs\BioInformatics\MEGA4. A2. In the MEGA4 window, go to Alignment|Alignment Explorer/CLUSTAL. Select ‘Create a new alignment’, and click on OK. Click on [NO] for protein sequence alignment. A3. Sequences can be entered either from FASTA files or by hand. We will ...
Rugby nutrition - Reading Crusade
... amino acids from our diets. Protein in the food we eat supplies these amino acids, which the body uses to make its own proteins. ...
... amino acids from our diets. Protein in the food we eat supplies these amino acids, which the body uses to make its own proteins. ...
Characterization of Rice Group 3 LEA Genes in Developmental
... (OsG3LEA-47.3, OsG3LEA-41.9, OsG3LEA-20.5, and OsG3LEA-24.5) that shared characteristics of canonical G3LEAs such as multiple copies of consensus motif, hydrophilic, structural intrinsic disorder, thermostability, abscisic acid (ABA)-responsiveness, and high G and C content in gene sequence. Under n ...
... (OsG3LEA-47.3, OsG3LEA-41.9, OsG3LEA-20.5, and OsG3LEA-24.5) that shared characteristics of canonical G3LEAs such as multiple copies of consensus motif, hydrophilic, structural intrinsic disorder, thermostability, abscisic acid (ABA)-responsiveness, and high G and C content in gene sequence. Under n ...
... 5. (8 pts) Sketch one regular secondary structure in the space to the right. Your drawing should provide: i) A representation of the mainchain shape. ii) An indication of the location of hydrogen bonds. iii) An indication of the location of the sidechains. iv) The name of the structure you have draw ...
Docking Studies in Target Proteins Involved in Antibacterial Action
... ganomycins and austrocortiluteins, these proteins may be involved as possible targets of such antimicrobial compounds. On the other hand, IARS revealed the highest scores for most of the 34 compounds tested, suggesting that IARS is not a good target for the tested compounds, specially taking into ac ...
... ganomycins and austrocortiluteins, these proteins may be involved as possible targets of such antimicrobial compounds. On the other hand, IARS revealed the highest scores for most of the 34 compounds tested, suggesting that IARS is not a good target for the tested compounds, specially taking into ac ...
Iron-Binding Activity of FutA1 Subunit of an ABC
... Synechocystis PCC 6803 does not possess a homologue of tbpA. Furthermore, our previous results indicated that simultaneous inactivation of four genes homologous to siderophore receptor genes had no effect on the uptake of iron and there has been no report on the production of siderophore by Synechoc ...
... Synechocystis PCC 6803 does not possess a homologue of tbpA. Furthermore, our previous results indicated that simultaneous inactivation of four genes homologous to siderophore receptor genes had no effect on the uptake of iron and there has been no report on the production of siderophore by Synechoc ...
Predicting functional linkages from gene fusions with
... is to identify ‘bidirectional best hits’ (for example, see Overbeek et al 1999): if the most similar sequence to protein A in genome 2 is B, and if the most similar sequence to protein B in genome 1 is A, then A and B are bidirectional best hits, and are operationally considered to be orthologues. I ...
... is to identify ‘bidirectional best hits’ (for example, see Overbeek et al 1999): if the most similar sequence to protein A in genome 2 is B, and if the most similar sequence to protein B in genome 1 is A, then A and B are bidirectional best hits, and are operationally considered to be orthologues. I ...
Quality Attributes of Biologics
... metabolism and is secreted by the ultimobranchial gland of salmon. It is produced from either synthetic processes or microbial processes using recombinant DNA (rDNA) technology. The host cell-derived protein content and the host cell- or vector-derived DNA content of Calcitonin Salmon produced from ...
... metabolism and is secreted by the ultimobranchial gland of salmon. It is produced from either synthetic processes or microbial processes using recombinant DNA (rDNA) technology. The host cell-derived protein content and the host cell- or vector-derived DNA content of Calcitonin Salmon produced from ...
Module 7 - Protein Structure Prediction
... sequence or functional similarity. Fortunately, certain folds crop up time and time again in proteins, and so fold recognition methods for predicting protein structure can be very effective. Methods of fold recognition attempt to detect similarities between the 3D structure of proteins that do not e ...
... sequence or functional similarity. Fortunately, certain folds crop up time and time again in proteins, and so fold recognition methods for predicting protein structure can be very effective. Methods of fold recognition attempt to detect similarities between the 3D structure of proteins that do not e ...
Nucleic Acid and Protein Quantitation Methods
... The color change of this assay is measured at 595 or 600 nm. The Bradford assay takes only 10 minutes but is TM sensitive to detergent which is often found in protein samples. Finally, the bicinchoninic acid (BCA ) method was ...
... The color change of this assay is measured at 595 or 600 nm. The Bradford assay takes only 10 minutes but is TM sensitive to detergent which is often found in protein samples. Finally, the bicinchoninic acid (BCA ) method was ...
Protein - people.vcu.edu
... called the active site. The folding happens to place the 195th amino acid in the chain, serine, near a hole that has the shape of the amino acid phenylalanine. When a phenylalanine within a protein you eat finds its way into the phenylalanine-shaped hole of chymotrypsin, the amide bond adjacent to p ...
... called the active site. The folding happens to place the 195th amino acid in the chain, serine, near a hole that has the shape of the amino acid phenylalanine. When a phenylalanine within a protein you eat finds its way into the phenylalanine-shaped hole of chymotrypsin, the amide bond adjacent to p ...
Kellen.Ian.Aminoacids
... Structure: Proteins are the chief constituents of skin, bones, hair, and nails for animals. Collagen and keratin are two important structural proteins. Catalysis: All reactions that take place in living organisms are catalyzed by proteins called enzymes. Without enzymes, the reaction would be so ...
... Structure: Proteins are the chief constituents of skin, bones, hair, and nails for animals. Collagen and keratin are two important structural proteins. Catalysis: All reactions that take place in living organisms are catalyzed by proteins called enzymes. Without enzymes, the reaction would be so ...
The Structure of Amino Acids in Proteins
... When amino acids are joined together, a peptide bond (see red line below) is made and water comes off. ...
... When amino acids are joined together, a peptide bond (see red line below) is made and water comes off. ...
Aspartic acid or Glutamic Acid Histidine
... Wild-type Protein shown on the right: Choice B: Explain the difference in entropy (∆So) between the wild-type protein and the Phe57→Val mutant, i.e. why is the overall entropy change larger for the mutant protein. The overall entropy change is higher because the non-polar surface area of Val is smal ...
... Wild-type Protein shown on the right: Choice B: Explain the difference in entropy (∆So) between the wild-type protein and the Phe57→Val mutant, i.e. why is the overall entropy change larger for the mutant protein. The overall entropy change is higher because the non-polar surface area of Val is smal ...
A single amino acid change, Q114R, in the cleavage
... mutations also have an I118V mutation. It should be noted that, in the hypothetical two-dimensional model of furin substrate binding-site domains, the enzymic subdomain of furin that interacts with glutamine and also with valine is not a distinct site and the substrate points away from the enzyme to ...
... mutations also have an I118V mutation. It should be noted that, in the hypothetical two-dimensional model of furin substrate binding-site domains, the enzymic subdomain of furin that interacts with glutamine and also with valine is not a distinct site and the substrate points away from the enzyme to ...
Infrared spectroscopy: a tool for protein characterization Chenge Li
... Proteins are relatively large, compact, structurally complex molecules that are made from small molecules called amino acids. Amino acids share a common structure, which contains an amino group (-NH2), a carboxyl group (-COOH), along with a side chain that is specific for each amino acid. The side c ...
... Proteins are relatively large, compact, structurally complex molecules that are made from small molecules called amino acids. Amino acids share a common structure, which contains an amino group (-NH2), a carboxyl group (-COOH), along with a side chain that is specific for each amino acid. The side c ...
15 Tacks and a 4 Foot Toober
... • And pairing green tacks that form disulfide bonds, • And keeping all of the polar white tacks on the surface of the protein. After everyone has folded their toober as best they can, the teacher can point out: • Every toober had a different random sequence of tacks (amino acids) and therefore each ...
... • And pairing green tacks that form disulfide bonds, • And keeping all of the polar white tacks on the surface of the protein. After everyone has folded their toober as best they can, the teacher can point out: • Every toober had a different random sequence of tacks (amino acids) and therefore each ...
Oligomerization and activation of the FliI ATPase
... whether the flagellar ATPase FliI can assemble to a multimeric state, if there might be a connection between such multimerization and enzymatic activation and whether this might be promoted by contact with phospholipid. ...
... whether the flagellar ATPase FliI can assemble to a multimeric state, if there might be a connection between such multimerization and enzymatic activation and whether this might be promoted by contact with phospholipid. ...
Investigation of the starch-binding properties of wheat friabilin
... The most important trait that determines how a particular wheat cultivar is processed is grain hardness (texture). Grain hardness forms the fundamental basis of commercial differentiation. It is possible to differentiate ‘soft’ and ‘hard’ hexaploid wheats and ‘very hard’ durum wheat as three distinc ...
... The most important trait that determines how a particular wheat cultivar is processed is grain hardness (texture). Grain hardness forms the fundamental basis of commercial differentiation. It is possible to differentiate ‘soft’ and ‘hard’ hexaploid wheats and ‘very hard’ durum wheat as three distinc ...
GPCRs10
... • Many receptors show constitutive activity even when expressed at physiol levels (e.g., rat dopamine D1, rat, human hist H2, human dopamine D3, and human 5-HT1A). • Inverse agonists. • Mutations have been identified that incr the basal activity w/o affecting the ability of agonists to further activ ...
... • Many receptors show constitutive activity even when expressed at physiol levels (e.g., rat dopamine D1, rat, human hist H2, human dopamine D3, and human 5-HT1A). • Inverse agonists. • Mutations have been identified that incr the basal activity w/o affecting the ability of agonists to further activ ...
It Works! Shake™ Product Info Sheet
... Soy-free, dairy-free, and Non-GMO, this plantbased protein makes It Works! Shake a great choice for vegans, vegetarians, or anyone with soy/dairy sensitivity or anyone who wants to ensure their protein is clean! What are the “plants” used in the plant-based protein of It Works! Shake? It Works! Shak ...
... Soy-free, dairy-free, and Non-GMO, this plantbased protein makes It Works! Shake a great choice for vegans, vegetarians, or anyone with soy/dairy sensitivity or anyone who wants to ensure their protein is clean! What are the “plants” used in the plant-based protein of It Works! Shake? It Works! Shak ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... blood fluke that occurs in China. It is the cause of Schistosomiasis japonica, a disease that still remains a significant health problem especially in lake and marshland regions. Schistosomiasis is an infection caused mainly by three schistosome species; Schistosoma mansoni, Schistosoma japonicum an ...
... blood fluke that occurs in China. It is the cause of Schistosomiasis japonica, a disease that still remains a significant health problem especially in lake and marshland regions. Schistosomiasis is an infection caused mainly by three schistosome species; Schistosoma mansoni, Schistosoma japonicum an ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.