Indian Agricultural Research Institute, New Delhi
... Lack of rapid and accurate analytical techniques for small quantities Negative correlation between grain protein content and yield Low genetic variability and requirement for accurate analysis The experience in maize - chalky endosperm, dull appearance and high moisture content of seeds -sus ...
... Lack of rapid and accurate analytical techniques for small quantities Negative correlation between grain protein content and yield Low genetic variability and requirement for accurate analysis The experience in maize - chalky endosperm, dull appearance and high moisture content of seeds -sus ...
Calcium Signaling
... 3’ untranslated region structure • Post-transcriptional control – 2° and 3° structure of mRNA – Analogous to DNA promoter ...
... 3’ untranslated region structure • Post-transcriptional control – 2° and 3° structure of mRNA – Analogous to DNA promoter ...
Targeted Identification of Glycoproteins in Disease
... While Ac4GlcNAz may not serve as a selective therapeutic target, I worked to explore it’s utility in studying the glycoproteins of bacteria which do incorporate it. Specifically, I worked towards identifying the glycoproteins of C. jejuni that have been labeled with Ac4GlcNAz. I employed “Click-iT” ...
... While Ac4GlcNAz may not serve as a selective therapeutic target, I worked to explore it’s utility in studying the glycoproteins of bacteria which do incorporate it. Specifically, I worked towards identifying the glycoproteins of C. jejuni that have been labeled with Ac4GlcNAz. I employed “Click-iT” ...
Understanding Tools and Techniques in Protein Structure Prediction
... bioinformatics, it has been made possible to understand the relationship between amino acid sequence and three-dimensional structure in proteins. The central challenge of bioinformatics is the rationalization of the mass of sequence information not only to derive efficient means of storage and retri ...
... bioinformatics, it has been made possible to understand the relationship between amino acid sequence and three-dimensional structure in proteins. The central challenge of bioinformatics is the rationalization of the mass of sequence information not only to derive efficient means of storage and retri ...
Steven`s project - The University of Texas at Dallas
... The uses of the software as well as sample command lines are given in the various README documents given in the Rosetta package. There are a few important things worth noting at this time to make use of the software run more smoothly. Firstly, Rosetta is not supported on all operating system platfor ...
... The uses of the software as well as sample command lines are given in the various README documents given in the Rosetta package. There are a few important things worth noting at this time to make use of the software run more smoothly. Firstly, Rosetta is not supported on all operating system platfor ...
Biochemical and Cellular Investigation of Vitreoscilla Hemoglobin
... Crude cell extracts were centrifuged at 13,000 ×g, 4oC for 15 min and the supernatant was collected. Before loading onto an affinity column, the supernatant was dissolved in lysis buffer (100 mM TrisHCl containing 0.5% Triton X-100, pH 8.0) and filtered through a 0.2-µm polycarbonate membrane. The p ...
... Crude cell extracts were centrifuged at 13,000 ×g, 4oC for 15 min and the supernatant was collected. Before loading onto an affinity column, the supernatant was dissolved in lysis buffer (100 mM TrisHCl containing 0.5% Triton X-100, pH 8.0) and filtered through a 0.2-µm polycarbonate membrane. The p ...
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins
... malting and brewing processes, whereas the modified LTP1 species is the most important one for the beer foam potential. Jégou and co-workers (2000) investigated the chemical modifications of barley LTP1 that occur during the malting and brewing process, by comparing the proteins isolated from seeds ...
... malting and brewing processes, whereas the modified LTP1 species is the most important one for the beer foam potential. Jégou and co-workers (2000) investigated the chemical modifications of barley LTP1 that occur during the malting and brewing process, by comparing the proteins isolated from seeds ...
Structural and functional features of Drosophila chorion proteins s36
... and more than 14 minor chorion proteins can be resolved by two-dimensional gel electrophoresis; subsets of these proteins are expressed in a temporally regulated mode during the 5 h of choriogenesis 5 7. The sequences of all major chorion proteins have been determined: the lower molecular weight s15 ...
... and more than 14 minor chorion proteins can be resolved by two-dimensional gel electrophoresis; subsets of these proteins are expressed in a temporally regulated mode during the 5 h of choriogenesis 5 7. The sequences of all major chorion proteins have been determined: the lower molecular weight s15 ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... then either to staining with Coomassie blue or to immunoblotting with specific antibodies against each of three major storage proteins. Figure 3 shows that the vesicles accumulated considerable amounts of three proteins that stained with Coomassie blue (lane 2). The immunoblot showed that these prot ...
... then either to staining with Coomassie blue or to immunoblotting with specific antibodies against each of three major storage proteins. Figure 3 shows that the vesicles accumulated considerable amounts of three proteins that stained with Coomassie blue (lane 2). The immunoblot showed that these prot ...
The role of carbohydrate in sperm
... number of different epitopes within the ZP3, i.e. gamete interaction is a process where recognition of gametes relies on multivalent ligand interactions. With regard to the molecular basis of cell adhesion, individual protein-carbohydrate interactions are thought to be very weak and often of broad s ...
... number of different epitopes within the ZP3, i.e. gamete interaction is a process where recognition of gametes relies on multivalent ligand interactions. With regard to the molecular basis of cell adhesion, individual protein-carbohydrate interactions are thought to be very weak and often of broad s ...
Structural Basis of Biological Nitrogen Fixation
... 170 × 109 kg of ammonia8). By definition, biological nitrogen fixation must be achieved under physiological conditions of ∼290 K and 0.8 atm N2 which, consequently, suggests a higher degree of sophistication in chemical catalysis. The lower reaction temperature of the enzymatic process not only impl ...
... 170 × 109 kg of ammonia8). By definition, biological nitrogen fixation must be achieved under physiological conditions of ∼290 K and 0.8 atm N2 which, consequently, suggests a higher degree of sophistication in chemical catalysis. The lower reaction temperature of the enzymatic process not only impl ...
Isolation and Characterization of Plastidic Glucose-6-Phosphate Dehydrogenase
... linked to nitrogen metabolism through the prerequisite of reductant (Emes and Bowsher, 1991) but its true involvement in fatty acid synthesis still remains poorly characterized. The first committed step of fatty acid synthesis occurs in the plastid with the formation of malonyI-CoA from acetyI-CoA a ...
... linked to nitrogen metabolism through the prerequisite of reductant (Emes and Bowsher, 1991) but its true involvement in fatty acid synthesis still remains poorly characterized. The first committed step of fatty acid synthesis occurs in the plastid with the formation of malonyI-CoA from acetyI-CoA a ...
Partial Class Notes Chapters 3 and 5 (4 slides/page)
... Proteins can also be separated by electrophoresis based on their native charge. ________________: pH at which net protein charge is zero ________________: electrophoresis of proteins (w/o SDS) in a pH gradient to a position in the gel at which pH = pI. pH gradient formed by polyampholytes (small mul ...
... Proteins can also be separated by electrophoresis based on their native charge. ________________: pH at which net protein charge is zero ________________: electrophoresis of proteins (w/o SDS) in a pH gradient to a position in the gel at which pH = pI. pH gradient formed by polyampholytes (small mul ...
BCMB 3100 – Chapter 3 (part 1)
... eluted with salt. Non-charged proteins and proteins of similar charge to resin will elute first. ...
... eluted with salt. Non-charged proteins and proteins of similar charge to resin will elute first. ...
Plasma Amino Acid Response to Graded Levels of Escape Protein
... The plasma amino acid broken-line technique can be used to identify limiting amino acids of ruminants if ruminal degradation of the supplemented amino acids is avoided (Fenderson and Bergen, 1975; Titgemeyer et al., 1986). However, the need to confine animals in metabolism stalls to enable abomasal ...
... The plasma amino acid broken-line technique can be used to identify limiting amino acids of ruminants if ruminal degradation of the supplemented amino acids is avoided (Fenderson and Bergen, 1975; Titgemeyer et al., 1986). However, the need to confine animals in metabolism stalls to enable abomasal ...
Urea cycle
... liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidney. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney and other organs function ...
... liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidney. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney and other organs function ...
¹⁵N Heteronuclear Single Quantum Coherence
... due to the extended conjugated π-electron system. There are often three absorption bands within the visible region, the α-band, the β-band, and the Soret band, which are in order of increasing energy. The Soret band, located in the 409-420nm region, is commonly ten times more intense than the α and ...
... due to the extended conjugated π-electron system. There are often three absorption bands within the visible region, the α-band, the β-band, and the Soret band, which are in order of increasing energy. The Soret band, located in the 409-420nm region, is commonly ten times more intense than the α and ...
The CamSol Method of Rational Design of Protein Mutants with
... thousands of amino acid substitutions or insertions to identify specific mutations that are predicted to maximally increase the solubility of a protein while preserving its fundamental properties, including its functional structure and binding affinity. The method requires the knowledge of the nativ ...
... thousands of amino acid substitutions or insertions to identify specific mutations that are predicted to maximally increase the solubility of a protein while preserving its fundamental properties, including its functional structure and binding affinity. The method requires the knowledge of the nativ ...
Bioinformatics approaches for functional
... energy coupling mechanism and substrate specificities [18,19]. They form an intricate system of pumps and channels through which they deliver essential nutrients, eject waste products and assist the cell to sense environmental conditions [20]. Ion channels are membrane proteins, which facilitate the ...
... energy coupling mechanism and substrate specificities [18,19]. They form an intricate system of pumps and channels through which they deliver essential nutrients, eject waste products and assist the cell to sense environmental conditions [20]. Ion channels are membrane proteins, which facilitate the ...
Descriptions of translation related genes that
... L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine bio ...
... L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine bio ...
bi6103-20feb04 - NUS School of Computing
... plasma membrane. • Endocytosis is the opposite of exocytosis and involves the uptake of extracellular material by pinching off vesicles from the plasma membrane • The contents of the endocytic vesicles are delivered to the lysosomes by membrane fusion ...
... plasma membrane. • Endocytosis is the opposite of exocytosis and involves the uptake of extracellular material by pinching off vesicles from the plasma membrane • The contents of the endocytic vesicles are delivered to the lysosomes by membrane fusion ...
Protein Structure - Macmillan Learning
... must first purify it from all other proteins in the cell. First, cells are lysed and particulate matter is removed by centrifugation, to yield a “crude extract.” The crude extract is then fractionated to separate the proteins and isolate the one that is of particular interest, using a variety of tec ...
... must first purify it from all other proteins in the cell. First, cells are lysed and particulate matter is removed by centrifugation, to yield a “crude extract.” The crude extract is then fractionated to separate the proteins and isolate the one that is of particular interest, using a variety of tec ...
The hnRNP C Proteins Contain a Nuclear Retention Sequence That
... between these possibilities, an expression plasmid encoding hnRNP C1 fused at its carboxyl terminus to hnRNP A1 was constructed, and the nucleocytoplasmic transport properties of the expressed fusion protein were examined using the heterokaryon assay (Fig. 1 A). The C1-A1 fusion protein behaved iden ...
... between these possibilities, an expression plasmid encoding hnRNP C1 fused at its carboxyl terminus to hnRNP A1 was constructed, and the nucleocytoplasmic transport properties of the expressed fusion protein were examined using the heterokaryon assay (Fig. 1 A). The C1-A1 fusion protein behaved iden ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.