Biological significance of structural differences between two highly
... (Fig. 1A), because it lies in proximity to a.a. 116–129 in the Uev1 molecular structure (Fig. 1C). The Uev1D30-F104S single amino acid substitution abrogated binding by LN2A, but did not affect ...
... (Fig. 1A), because it lies in proximity to a.a. 116–129 in the Uev1 molecular structure (Fig. 1C). The Uev1D30-F104S single amino acid substitution abrogated binding by LN2A, but did not affect ...
Single-stranded heteroduplex intermediates in l Red homologous
... target BACs of at least 50 kb at about the same efficiency as small deletions, the converse event of insertion is very sensitive to increasing size. Insertions up to 3 kb are most efficiently achieved using beta recombination, however at greater sizes, an alternative Red-mediated mechanism(s) appear ...
... target BACs of at least 50 kb at about the same efficiency as small deletions, the converse event of insertion is very sensitive to increasing size. Insertions up to 3 kb are most efficiently achieved using beta recombination, however at greater sizes, an alternative Red-mediated mechanism(s) appear ...
1. True or False? The standard human karotype consists of 23 pairs
... between species – is a major process in the evolution of higher plants. True ...
... between species – is a major process in the evolution of higher plants. True ...
Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1a/tcf1
... genes were shown to be expressed abundantly in liver, pancreas, gut and kidney. Zebrafish HNF1a and HNF1h proteins contain all HNF1 signature domains including the dimerization domain, POU-like domain and atypical homeodomain. Sequence and phylogenetic analysis reveals that zebrafish hnf1a is closer ...
... genes were shown to be expressed abundantly in liver, pancreas, gut and kidney. Zebrafish HNF1a and HNF1h proteins contain all HNF1 signature domains including the dimerization domain, POU-like domain and atypical homeodomain. Sequence and phylogenetic analysis reveals that zebrafish hnf1a is closer ...
CIBI3031-091 Midterm Examination III November 2005
... ____ 36. Red-green color blindness is an X-linked recessive trait in humans. A color-blind woman and a man with normal vision have a son. What is the probability that the son is color blind? a. 100 percent b. 50 percent c. 75 percent ____ 37. If ALL offspring of a cross have the genotype Aa, the par ...
... ____ 36. Red-green color blindness is an X-linked recessive trait in humans. A color-blind woman and a man with normal vision have a son. What is the probability that the son is color blind? a. 100 percent b. 50 percent c. 75 percent ____ 37. If ALL offspring of a cross have the genotype Aa, the par ...
CIBI3031-070 Midterm Examination III November 2005
... b. somatic cells c. reduction of the chromosome number ____ 24. If a daughter expresses an X-linked recessive gene, she inherited the trait from a. her mother. b. both parents. c. her father. ____ 25. If two genes are on the same chromosome, a. they are in the same linkage group. b. they assort inde ...
... b. somatic cells c. reduction of the chromosome number ____ 24. If a daughter expresses an X-linked recessive gene, she inherited the trait from a. her mother. b. both parents. c. her father. ____ 25. If two genes are on the same chromosome, a. they are in the same linkage group. b. they assort inde ...
Malate Dehydrogenase
... The loop is highly conserved among MDHs (residues 98-110), reflecting its crucial role in catalysis (Fig. 1). X-ray structures of MDHs crystallized in the presence and absence of substrate analogues, which bind to the active site, have identified 2 conformationally distinct forms of the enzyme with ...
... The loop is highly conserved among MDHs (residues 98-110), reflecting its crucial role in catalysis (Fig. 1). X-ray structures of MDHs crystallized in the presence and absence of substrate analogues, which bind to the active site, have identified 2 conformationally distinct forms of the enzyme with ...
as a PDF
... (Yang, 1986; Yu and Yang, 1993) . The preparations of kinase FA phosphorylated myelin basic protein with a specific activity of - 1,600 nmol/min/mg and with a specific activity of 26,000 nmol/min/mg toward activation of inactive type1 protein phosphatase . When analyzed by SDS-PAGE and Coomassie Blu ...
... (Yang, 1986; Yu and Yang, 1993) . The preparations of kinase FA phosphorylated myelin basic protein with a specific activity of - 1,600 nmol/min/mg and with a specific activity of 26,000 nmol/min/mg toward activation of inactive type1 protein phosphatase . When analyzed by SDS-PAGE and Coomassie Blu ...
Farnesyl diphosphate synthase, the target for nitrogen
... The mevalonate pathway of isoprenoid biosynthesis allows eukaryotic cells to convert acetyl-CoA into FDP (farnesyl diphosphate). FDP is mainly used for biosynthesis of sterols but it may also be used, either directly or after conversion into geranylgeranyl diphosphate, for protein prenylation [1]. T ...
... The mevalonate pathway of isoprenoid biosynthesis allows eukaryotic cells to convert acetyl-CoA into FDP (farnesyl diphosphate). FDP is mainly used for biosynthesis of sterols but it may also be used, either directly or after conversion into geranylgeranyl diphosphate, for protein prenylation [1]. T ...
- Wiley Online Library
... triggering the movement of sister chromatids to opposite poles of the cell during anaphase. Chromosomes are symbolized by DNA double helices. ...
... triggering the movement of sister chromatids to opposite poles of the cell during anaphase. Chromosomes are symbolized by DNA double helices. ...
Transamination and Reductive Amination
... NADH or NADPH as cofactor. The synthetic usefulness of the transaminase reaction is diminished by the position of the equilibrium (Keq is often close to 1), which means that either complex mixtures result that are often laborious to separate, or extra measures are required to shift the equilibrium c ...
... NADH or NADPH as cofactor. The synthetic usefulness of the transaminase reaction is diminished by the position of the equilibrium (Keq is often close to 1), which means that either complex mixtures result that are often laborious to separate, or extra measures are required to shift the equilibrium c ...
Imprinting and Seed Development
... Lin (1982) also distinguished specific maize chromosome arms that had a parent-specific effect on seed size. Seeds with endosperm that lacked part of a chromosome or contained it in duplicate were created by reciprocal translocations between standard chromosomes and the accessory B chromosomes, whic ...
... Lin (1982) also distinguished specific maize chromosome arms that had a parent-specific effect on seed size. Seeds with endosperm that lacked part of a chromosome or contained it in duplicate were created by reciprocal translocations between standard chromosomes and the accessory B chromosomes, whic ...
medical chemistry and biochemistry
... protein structure and indicate the present state of knowledge concerning the stepwise process by which protein are thought to attain their native conformation. 5. Identify the physiologic roles of chaperones in protein maturation. Describe multiple chromatographic methods commonly employed for the i ...
... protein structure and indicate the present state of knowledge concerning the stepwise process by which protein are thought to attain their native conformation. 5. Identify the physiologic roles of chaperones in protein maturation. Describe multiple chromatographic methods commonly employed for the i ...
Chapter 25: Urinary System
... Protein Anabolism • Production of new proteins by formation of peptide bonds between amino acids – 10 essential amino acids are ones we must eat because we can not synthesize them – nonessential amino acids can be synthesized by transamination (transfer of an amino group to a substance to create an ...
... Protein Anabolism • Production of new proteins by formation of peptide bonds between amino acids – 10 essential amino acids are ones we must eat because we can not synthesize them – nonessential amino acids can be synthesized by transamination (transfer of an amino group to a substance to create an ...
A Fitness-Independent Evolvability Measure for Evolutionary
... comparing TF dynamics in one cell and therefore at only one point in the environment. If there are more than one cell or the number of cells of two developmental process differs, which is almost always true in cellular growth, it is hardly possible to calculate the phenotype distance. Thus, this phe ...
... comparing TF dynamics in one cell and therefore at only one point in the environment. If there are more than one cell or the number of cells of two developmental process differs, which is almost always true in cellular growth, it is hardly possible to calculate the phenotype distance. Thus, this phe ...
Metabolic fate and effects of saturated and unsaturated fatty acids in
... In the presence of both acetate and myristate glucose utilization was unaltered. On the other hand, glucose utilization was significantly reduced when cells were exposed to either palmitate, oleate, linoleate or arachidonate. DISCUSSION The fatty acids used in the present study are readily extracted ...
... In the presence of both acetate and myristate glucose utilization was unaltered. On the other hand, glucose utilization was significantly reduced when cells were exposed to either palmitate, oleate, linoleate or arachidonate. DISCUSSION The fatty acids used in the present study are readily extracted ...
Effect of Alanine-293 Replacement on the Activity, ATP Binding, and
... leucylation of tRNALeu. Several mutants in the CP1 domain of Escherichia coli LeuRS were obtained by introduction of restriction endonuclease sites into its gene, leuS. Of these mutants, only LeuRS-A293F had decreased activity (46%) compared to the native enzyme. To investigate the effect of A293 on ...
... leucylation of tRNALeu. Several mutants in the CP1 domain of Escherichia coli LeuRS were obtained by introduction of restriction endonuclease sites into its gene, leuS. Of these mutants, only LeuRS-A293F had decreased activity (46%) compared to the native enzyme. To investigate the effect of A293 on ...
C urrent and prospective applications of metal ion–protein
... proteins is largely governed by exposed histidine residues on the protein surface [52,58]. Cysteine also displays strong metal affinity, although to a somewhat lesser extent than histidine [33,38,59]. The metal affinity of these two amino acids can be largely attributed to their functional groups, i ...
... proteins is largely governed by exposed histidine residues on the protein surface [52,58]. Cysteine also displays strong metal affinity, although to a somewhat lesser extent than histidine [33,38,59]. The metal affinity of these two amino acids can be largely attributed to their functional groups, i ...
Patent constraints
... 1. An isolated nucleic acid molecule comprising a nucleotide sequence which (i) encodes a protein or polypeptide comprising an amino acid sequence of SEQ. ID. No. 3, SEQ. ID. No. 5, SEQ. ID. No. 7, SEQ. ID. No. 9, SEQ. ID. No. 11, SEQ. ID. No. 13, SEQ. ID. No. 15, SEQ. ID. No. 17, SEQ. ID. No. 20, S ...
... 1. An isolated nucleic acid molecule comprising a nucleotide sequence which (i) encodes a protein or polypeptide comprising an amino acid sequence of SEQ. ID. No. 3, SEQ. ID. No. 5, SEQ. ID. No. 7, SEQ. ID. No. 9, SEQ. ID. No. 11, SEQ. ID. No. 13, SEQ. ID. No. 15, SEQ. ID. No. 17, SEQ. ID. No. 20, S ...
A genetically encoded fluorescent tRNA is active in live
... despite each having a well-defined tertiary structure, enables the tRNA to perform live-cell protein synthesis in Escherichia coli. This tRNA–aptamer fusion can be switched on with quantitative GFP (green fluorescence protein)-like fluorescence, while acting like a canonical tRNA. Most importantly, ...
... despite each having a well-defined tertiary structure, enables the tRNA to perform live-cell protein synthesis in Escherichia coli. This tRNA–aptamer fusion can be switched on with quantitative GFP (green fluorescence protein)-like fluorescence, while acting like a canonical tRNA. Most importantly, ...
Centromere-Linkage Analysis and Consolidation
... separating two letters indicates that the markeris codominant in the two genetic backgrounds shown (Z.P., the locus produces slightly different s i x d PCR products from the different alleles, and haploid individuals in the mapping panel have one or the other sized product, but never both and never ...
... separating two letters indicates that the markeris codominant in the two genetic backgrounds shown (Z.P., the locus produces slightly different s i x d PCR products from the different alleles, and haploid individuals in the mapping panel have one or the other sized product, but never both and never ...
Evolution of Functionally Diverse Alleles
... genetics.bwh.harvard.edu/pph2/) to predict which nonsynonymous substitutions change protein function. PolyPhen-2 infers whether an amino acid change is ‘‘benign,’’ ‘‘possibly damaging,’’ or ‘‘probably damaging’’ based on evolutionary and structural information (Ramensky et al. 2002). ...
... genetics.bwh.harvard.edu/pph2/) to predict which nonsynonymous substitutions change protein function. PolyPhen-2 infers whether an amino acid change is ‘‘benign,’’ ‘‘possibly damaging,’’ or ‘‘probably damaging’’ based on evolutionary and structural information (Ramensky et al. 2002). ...
Costimulatory receptors in jawed vertebrates: Conserved
... (Tetraodon nigroviridis) and zebrafish (Danio rerio). In zebrafish, it was not possible to find sequences corresponding to exons 3 and 4 (encoding transmembrane and intracytoplasmic region) from the available genome assembly. The Ensembl zebrafish genome assembly (v36) had two copies of CD28 exons 1 and ...
... (Tetraodon nigroviridis) and zebrafish (Danio rerio). In zebrafish, it was not possible to find sequences corresponding to exons 3 and 4 (encoding transmembrane and intracytoplasmic region) from the available genome assembly. The Ensembl zebrafish genome assembly (v36) had two copies of CD28 exons 1 and ...
- CSHL Institutional Repository
... an equational division instead of a reductional one, indicating that meiosis 1 is replaced by a mitotic division (Golubovskaya and Mashnenkov, 1975). In the el1 mutant, meiosis 2 is affected leading to the production of unreduced spores (Rhoades, 1956). Given the differences in form and function bet ...
... an equational division instead of a reductional one, indicating that meiosis 1 is replaced by a mitotic division (Golubovskaya and Mashnenkov, 1975). In the el1 mutant, meiosis 2 is affected leading to the production of unreduced spores (Rhoades, 1956). Given the differences in form and function bet ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.