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A first attempt to elucidate the amino acid sequence of some lichen
A first attempt to elucidate the amino acid sequence of some lichen

... Lichens are symbiotic associations between a fungus and a cyanobacterium (cyano-lichens) or a green alga (chlorolichens), joined to form a new biological entity different from its individual components. Recognition mechanisms used by lichens are based on the production and secretion of fungal lectin ...
Analyzing Amino-Acid Sequences to Determine Evolutionary
Analyzing Amino-Acid Sequences to Determine Evolutionary

... divergingfrom a common ancestor,the greaterthe differencesthat can be expectedin the amino-acid sequencesof their proteins. Twoproteins are commonly studied in attempting to deduce evolutionaryrelationships from differencesin amino-acid sequences.One is cytochromec, and the other ishemoglobin. Cytoc ...
Elucidating Substrate and Inhibitor Binding Sites on the Surface of
Elucidating Substrate and Inhibitor Binding Sites on the Surface of

... Protein kinases and phosphorylation cascades are essential for life, and they play key roles in the regulation of many cellular processes including cell proliferation, cell cycle progression, metabolic homeostasis, transcriptional activation and development.1–4 Aberrant regulation of protein phospho ...
Enzyme Mechanisms
Enzyme Mechanisms

... unrelated settings Subtilisin: externals very different from mammalian serine proteases; triad same ...
- Wiley Online Library
- Wiley Online Library

... mutant (Table 1), indicating that these His residues only partially participate in P14K function. In addition, residue Arg96, which is conserved among the self-subunit swapping chaperones (Fig. 2), was also changed to investigate any effect on P14K function. The purified mutant NHase encoded by AB-s ...
Ovid: Parenteral administration of different amounts of branch
Ovid: Parenteral administration of different amounts of branch

... Altered protein metabolism during injury has been well documented [1-3]. It has been suggested that total parenteral nutrition decreases high muscle protein breakdown and improves impaired body protein synthesis during sepsis [4-6]. The properties of the branch-chain amino acids (valine, leucine, is ...
Unit 1 Life Processes and Biochemistry
Unit 1 Life Processes and Biochemistry

...  Examples: enzymes, hemoglobin, melanin, keratin, insulin  How to identify: each amino acid has a N! ...
types of gel - WordPress.com
types of gel - WordPress.com

... a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resulting gel can be stored in a plastic bag in a refrigerator. ...
Biology: Life on Earth
Biology: Life on Earth

... repressor protein bound to operator, overlaps promoter free repressor proteins When lactose is not present, repressor proteins bind to the operator of the lactose operon. When RNA polymerase binds to the promoter, the repressor protein blocks access to the structural genes, which therefore cannot be ...
TRANSLATION: How to make proteins?
TRANSLATION: How to make proteins?

... • Drugs (chloramphenicol) that inhibit PT bind to the 25S rRNA (PT loop) • Mutations that provide resistance to these drugs map to the PT loop • Nearly all (99%) of proteins can be stripped from the large subunit and it still retains the PT activity • Only RNA chains are close enough to the PT cente ...
Feeding Lysine: A Nutritionist and Dairy Producer`s Perspective
Feeding Lysine: A Nutritionist and Dairy Producer`s Perspective

... Lysine is a basic AA, which means it bears a positive charge at physiological pH. As a result, its ε-amino group side chain easily forms covalent bonds with other AA and reducing sugars known as the Maillard reaction. Lysine’s basic side chain is so reactive that even when it is part of a peptide or ...
Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in
Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in

(a) A(1) - at www.arxiv.org.
(a) A(1) - at www.arxiv.org.

... nitrogen-atom numbers of nucleobases in a rearranged genetic code, codons for A, V, T, G and P form a crossed-intersection core (Fig. 1a); second, a stereostructural re-classification of the 20 amino acids lead to five distinct groups of amino acids (Scheme 1b), which precisely overlap the above fiv ...
Peer-reviewed Article PDF
Peer-reviewed Article PDF

... for protozoan cells proliferation and considered as essential nutrients for the protozoan parasites. For example, Neurospora crassa mutants lacking ODC activity cannot grow unless putrescine, spermidine or spermines were added to the growing media [12,13]. The protozoan pathogens, Trypanosomatids de ...
Exploring Prostate Proteins and Antibodies
Exploring Prostate Proteins and Antibodies

... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...
responses of cultured cardiac myocytes to lysosomotropic
responses of cultured cardiac myocytes to lysosomotropic

... bases failed to enhance acid proteinase secretion into the culture medium but apparently inactivated cellular cathepsin B activity. In contrast, methylated amino acids induced no alterations in acid proteinase activity or the cellular distribution of the two proteolytic enzymes. Lastly, weak bases m ...
Biologically active octapeptides
Biologically active octapeptides

... tion, the fragments can be easily dispersed into single groups to be employed in preparing the compounds of cells by repeated suction and expulsion from a Gilson this invention remains a matter well within one ordinar Pipetman. After 30 to 60 Pipetman operations, the tissue ily skilled in the art, i ...
Chem 410 Chapter 11: Polyprotic Acids and Bases Part 1 How
Chem 410 Chapter 11: Polyprotic Acids and Bases Part 1 How

... But these amino acid zwitterions are salts, actually internal salts. So they will have many physical properties in common with salts. So they are crystals with high melting points, are highly water soluble (but low solubilities in organic compounds), and they have large dipole moments. If in the ami ...
Bitis gabonica rhinoceros
Bitis gabonica rhinoceros

... rhinocerase, has been isolated and characterised from the venom of Bitis gabonica rhinoceros using liquid phase isoelectric focusing and gel filtration. Like many viper venom serine proteases, this enzyme is glycosylated; the estimated molecular mass of the native enzyme is approximately 36kDa, whic ...
Probabilities and Probabilistic Models
Probabilities and Probabilistic Models

... www.bioalgorithms.info ...
Proteasome inhibition induces reversible impairments in protein
Proteasome inhibition induces reversible impairments in protein

... (mRNA) into the different proteins necessary for cell homeostasis. The rate of protein synthesis can be regulated at many different levels ranging from alterations in the levels of ribosome complexes, altering ribosomal performance, and altering the availability of RNA molecules. An increasing numbe ...


... a) the temperature at which 50% of a DNA molecule is denatured. b) the temperature at which 50% of a protein molecule is denatured. c) the temperature at which membranes are 50% fluid. d) all of the above. 2. Which of the following statements is incorrect about most DNA polymerases a) require a prim ...
A Guide to Baking Enzymes
A Guide to Baking Enzymes

... “Enzyme active preparations” are permitted for use in baked goods with specific standards of identity under 21 CFR 136.110 through 136.180. This includes regular, enriched, milk, raisin, and whole wheat bread, rolls, and buns. Enzymes are exempted from ingredient labeling when they are used as “proc ...
Introduction to Enzymes - Rose
Introduction to Enzymes - Rose

... Although RNA can have catalytic activity, proteins have much more diverse chemistry, because proteins are comprised of 20 amino acids instead of only four nucleotides. In addition, the amino acids have several functional groups that are not present in nucleotides. The chemistry of the amino acid sid ...
Nonenzymatic, Self-Elimination Degradation
Nonenzymatic, Self-Elimination Degradation

... sodium phosphate buffer). We found that the rate of hydrolysis was unaffected in all three cases of pH (Fig. 4, d – f, and Fig. 5, b). To study the effect of the ionic strength on degradation, similar studies were performed using 20 mm sodium phosphate buffer. It was observed that different ionic st ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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