Arg305 of Streptomyces l-glutamate oxidase plays a crucial role for
... tested by the MBTH method with 0.5 mM L-tyrosine, 1 mM L-tryptophan and L-aspartate, 5 mM L-leucine, and 10 mM other substrates with the purified enzymes. Effects of pH on activity were examined using Britton-Robinson buffer at a pH range of 4.0–12.0. The activities were measured by the MBTH method a ...
... tested by the MBTH method with 0.5 mM L-tyrosine, 1 mM L-tryptophan and L-aspartate, 5 mM L-leucine, and 10 mM other substrates with the purified enzymes. Effects of pH on activity were examined using Britton-Robinson buffer at a pH range of 4.0–12.0. The activities were measured by the MBTH method a ...
Dual Location of the Mitochondrial Preprotein
... complex (Klodmann et al., 2011; Rode et al., 2011). A comparison of B14.7, Tim23-2, and yeast Tim23 shows that B14.7 is similar to yeast Tim23 in terms of overall characteristics and contains an Arg residue in the correct position of the PRAT consensus domain, G/AX2F/YX10RX3DX6G/A/SGX3G, compared wi ...
... complex (Klodmann et al., 2011; Rode et al., 2011). A comparison of B14.7, Tim23-2, and yeast Tim23 shows that B14.7 is similar to yeast Tim23 in terms of overall characteristics and contains an Arg residue in the correct position of the PRAT consensus domain, G/AX2F/YX10RX3DX6G/A/SGX3G, compared wi ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... understood [5, 6], but little biochemical and molecular genetic information exists on pollen functions. Some recent studies have tried to break the bottleneck. Transcriptomic analyses of Arabidopsis pollen have identified some transcripts expressed preferentially in pollen [7,8]. Functional classifi ...
... understood [5, 6], but little biochemical and molecular genetic information exists on pollen functions. Some recent studies have tried to break the bottleneck. Transcriptomic analyses of Arabidopsis pollen have identified some transcripts expressed preferentially in pollen [7,8]. Functional classifi ...
Microbiology 146:
... described previously (Encarnacio! n et al., 1995). Amino acids were quantified fluorimetrically using the pre-column derivation technique with 9-fluoroenylmethyl-chloroformate and a Nova-Pack C18 column (Waters ; 3n9 i.d.i150 mm) (Cevallos et al., 1996). Amino acid separation was performed with a 20 ...
... described previously (Encarnacio! n et al., 1995). Amino acids were quantified fluorimetrically using the pre-column derivation technique with 9-fluoroenylmethyl-chloroformate and a Nova-Pack C18 column (Waters ; 3n9 i.d.i150 mm) (Cevallos et al., 1996). Amino acid separation was performed with a 20 ...
Ribosomes of Mouse Liver following
... was further investigated. For this purpose the distribution of added free monomers, prelabeled with I4C-labeled orotic acid, was studied in the gradient used for separating free and membrane-bound ribosomes. Less than 5% of the added radioactive monomers were retained in the membranebound fraction e ...
... was further investigated. For this purpose the distribution of added free monomers, prelabeled with I4C-labeled orotic acid, was studied in the gradient used for separating free and membrane-bound ribosomes. Less than 5% of the added radioactive monomers were retained in the membranebound fraction e ...
Solution Blowing of Soy Protein Fibers
... containing some beads is shown in Figure 2b. It is emphasized that the blend of sp/nylon-6 blown to form the fibers used in Figure 2 was absolutely clear, which is an indication that it was homogeneous and no undissolved protein grains were present. When such a blend is strongly stretched during the ...
... containing some beads is shown in Figure 2b. It is emphasized that the blend of sp/nylon-6 blown to form the fibers used in Figure 2 was absolutely clear, which is an indication that it was homogeneous and no undissolved protein grains were present. When such a blend is strongly stretched during the ...
The Minimal Nutritional Requirements of Organisms
... species does not ferment glucose, In simple mixtures of amino acids glutamic acid was essential; for example, the strains would grow in a mixture of glutamic acid, proline and leucine but would not grow with proline and leucine alone. The growth requirements of the remaining six strains were more co ...
... species does not ferment glucose, In simple mixtures of amino acids glutamic acid was essential; for example, the strains would grow in a mixture of glutamic acid, proline and leucine but would not grow with proline and leucine alone. The growth requirements of the remaining six strains were more co ...
Systematic Structure-Function Analysis of the Small GTPase Arf1 in Yeast.
... to ArfGAP (Goldberg, 1999) were used for modeling Arf1p mutations. The “A” monomer of the Arf1-GDP dimer (protein database code IHUR) (Amor et al., 1994) was used for figures of the monomer. It should be noted that an N-terminal truncation Arf1 protein (⌬1– 17) was used to obtain the crystal structu ...
... to ArfGAP (Goldberg, 1999) were used for modeling Arf1p mutations. The “A” monomer of the Arf1-GDP dimer (protein database code IHUR) (Amor et al., 1994) was used for figures of the monomer. It should be noted that an N-terminal truncation Arf1 protein (⌬1– 17) was used to obtain the crystal structu ...
Introduction
... side chains of specific amino acid residues and also can be produced by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produced by any compound having at least 2 peptide linkages in its mole ...
... side chains of specific amino acid residues and also can be produced by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produced by any compound having at least 2 peptide linkages in its mole ...
Database Searching for Protein Identification and
... molecular mass constraint. When this is done, the tag is effectively allowed to float within the candidate sequence, so that a match is possible even if there is a difference in the calculated mass to one side or the other of the tag. This is one of the few ways of getting a match to a peptide when ...
... molecular mass constraint. When this is done, the tag is effectively allowed to float within the candidate sequence, so that a match is possible even if there is a difference in the calculated mass to one side or the other of the tag. This is one of the few ways of getting a match to a peptide when ...
Protein Folding and Expression
... proteases, which facilitates production of intact protein products. Examples of successfully expressed proteins can be seen in Table 1. This includes expression of enzymes, antigens, and cytokines. Each protein was produced at a very high level of expression and confirmed to have native biological a ...
... proteases, which facilitates production of intact protein products. Examples of successfully expressed proteins can be seen in Table 1. This includes expression of enzymes, antigens, and cytokines. Each protein was produced at a very high level of expression and confirmed to have native biological a ...
interaction analysis of aspirin with selective amino acids
... rate the possible interaction between AA and aspirin. It is evident from results that the absorbance of aspirin increased with the addition of amino acids and this increase was significant (p < 0.05). This increase in absorbance represents the increase in the ionization and thus enhanced solubility ...
... rate the possible interaction between AA and aspirin. It is evident from results that the absorbance of aspirin increased with the addition of amino acids and this increase was significant (p < 0.05). This increase in absorbance represents the increase in the ionization and thus enhanced solubility ...
Modeling tRNA*s Translator Function
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
Chapter 6 Identifying and Measuring Transmembrane Helix–Helix
... aspect of cell biology and physiology (1). Consequently, proper functioning of these proteins is vital to health, and specific defects are associated with many known human diseases (2, 3). Most are anchored to the cellular membrane through one or several transmembrane (TM) domains that predominantly ...
... aspect of cell biology and physiology (1). Consequently, proper functioning of these proteins is vital to health, and specific defects are associated with many known human diseases (2, 3). Most are anchored to the cellular membrane through one or several transmembrane (TM) domains that predominantly ...
Nitrogen Cycle
... converts into two separate atoms by undergoing some changes which combine nitrogen with Hydrogen. ...
... converts into two separate atoms by undergoing some changes which combine nitrogen with Hydrogen. ...
GST II: ---Title--- - Digital Biology Laboratory
... Step 1: Identify regions of the sequences with the highest density of matches. In this step exact matches of a given length (by default 2 for proteins, 6 for nucleic acids) are determined and regions (fragments of diagonals) with a high number of matches ...
... Step 1: Identify regions of the sequences with the highest density of matches. In this step exact matches of a given length (by default 2 for proteins, 6 for nucleic acids) are determined and regions (fragments of diagonals) with a high number of matches ...
Chapter 05
... group the (the N-terminus) and at the other is an amino acid with a free carboxyl group the (the Cterminus). ...
... group the (the N-terminus) and at the other is an amino acid with a free carboxyl group the (the Cterminus). ...
Comparative Analysis of Prothrombin Activators
... is released upon activation. The presence of a vitamin K– dependent, c-carboxyglutamic acid residue–rich region at the N-terminus of the light chain plays a critical role in the binding of phospholipids and calcium ions, while the heavy chain contains the catalytic site residues (Jackson 1984; Perss ...
... is released upon activation. The presence of a vitamin K– dependent, c-carboxyglutamic acid residue–rich region at the N-terminus of the light chain plays a critical role in the binding of phospholipids and calcium ions, while the heavy chain contains the catalytic site residues (Jackson 1984; Perss ...
The exam is worth 200 points, divided into 7 questions. You must do
... concerns the structure, folding, and function of an essential, tetrameric, allosteric enzyme which binds inorganic phosphate, Pi. The table below shows some of the amino acids from the human, rat, and fruit fly enzymes. In addition, mutants (indicated by the asterisks) have been engineered (via site ...
... concerns the structure, folding, and function of an essential, tetrameric, allosteric enzyme which binds inorganic phosphate, Pi. The table below shows some of the amino acids from the human, rat, and fruit fly enzymes. In addition, mutants (indicated by the asterisks) have been engineered (via site ...
Neonatal Parenteral Nutrition - UCSF Benioff Children`s Hospital
... • Nutrition support should be initiated within 3 days of birth and should include protein. A "starter TPN" solution is available to start TPN at arrival to the unit. • Start PN when at least 30 cc/kg/d can be used for this route. • Although growth can be obtained with PN, enteral feedings should be ...
... • Nutrition support should be initiated within 3 days of birth and should include protein. A "starter TPN" solution is available to start TPN at arrival to the unit. • Start PN when at least 30 cc/kg/d can be used for this route. • Although growth can be obtained with PN, enteral feedings should be ...
Kidney Function Testing
... The tubular and interstitial diseases affecting the kidney can be divided into those that produce acute and chronic disease: Acute tubulointerstitial disorders as acute tubular necrosis Chronic tubulointerstitial disorders as polycystic kidney disease ...
... The tubular and interstitial diseases affecting the kidney can be divided into those that produce acute and chronic disease: Acute tubulointerstitial disorders as acute tubular necrosis Chronic tubulointerstitial disorders as polycystic kidney disease ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.