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Ben Good.
Ben Good.

... • A given codon can either stand for a specific amino acid or act as a “start/stop codon”, which signals either the beginning or end of a protein’s code respectively. • There are 4*4*4=64 different codons but only 20 amino acids to code for, making a total of 21 different possible meanings for a giv ...
Genetics 314 – Spring, 2005
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A VIEW OF GENETICS.
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The genetics of cystic fibrosis
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MUTATIONS
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... 7. Cut out the 5 tRNA (transfer RNA) molecules. tRNA are found in the cytoplasm. On one side they have 3 nucleotide bases (called the anticodon). On their other side they have a receptor site for a particular amino acid. (For example the CAA anticodon codes for Valine only.) This tRNA would never pi ...
Cannus stannous: A Study of Evolution by Means of Natural Selection
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... Rett syndrome (RTT) is a neurodevelopmental disorder that almost exclusively affects girls. It is caused by mutations in the MECP2 gene that encodes the methyl-CpG-binding protein 2 (MeCP2). In this study we correlated mutation type and location with the severity of the phenotype in 123 girls with R ...
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... • 1. RNA = Ribose sugar instead of Deoxyribose in DNA • 2. DNA A-T C-G RNA A-U C-G • 3. RNA= Single stranded instead of Double in DNA • 4. RNA can go in and out of nucleus, DNA must stay in nucleus • 5. DNA can repair itself, RNA cannot ...
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Ch 4 Extensions of Mendelian Genetics
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... but nuclear genomes are identical – Symptoms of neurodegenerative diseases or other mutations may manifest in one twin, but not other. – In a heteroplasmic mother, chance of phenotype of offspring depends on both partitioning of mutant mtDNA after fertilization, and tissue that receives mutation dur ...
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... 2. Prenatal testing suggests the presence of OI symptoms in the fetus. In both situations, the woman and her partner should seek advice from a geneticist. Osteogenesis imperfecta is the result of a mutation in one of the two genes that carry instructions for making type 1 collagen -- the major prote ...
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GENE EXPRESSION AT THE MOLECULAR LEVEL

... Leder’s experiment to identify introns in the β-globin gene  Double stranded DNA of the β-globin gene was first separated into single strands and then mixed with mature mRNA of the βglobin gene  If mRNA binds to a region of a gene with one intron, two single stranded DNA loops will form that are s ...
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... •Given a multiple fasta sequence file*, write a script that for each sequence extract the gi number and the species name. and rewrites the file so that the annotation line starts with the gi number, followed by the species/strain name, followed by a space. (The gi number and the species name should ...
No Slide Title
No Slide Title

... – Each iteration involves propagating all elements of the population – Each member of population (“chromosome”) corresponds to one value of  • Genetic algorithms (GAs) are most popular form of EC • Early work in 1950s and 1960s; influential 1975 book by John Holland laid foundation for modern imple ...
gene-expression-text
gene-expression-text

... Introns: part of the gene which is transcribed but NOT found in the mRNA. CHMI 2227 up - E.R. Ph.D. after transcription. Exons: part of the gene which ends inGauthier, the mRNA ...
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Frameshift mutation



A frameshift mutation (also called a framing error or a reading frame shift) is a genetic mutation caused by indels (insertions or deletions) of a number of nucleotides in a DNA sequence that is not divisible by three. Due to the triplet nature of gene expression by codons, the insertion or deletion can change the reading frame (the grouping of the codons), resulting in a completely different translation from the original. The earlier in the sequence the deletion or insertion occurs, the more altered the protein. A frameshift mutation is not the same as a single-nucleotide polymorphism in which a nucleotide is replaced, rather than inserted or deleted. A frameshift mutation will in general cause the reading of the codons after the mutation to code for different amino acids. The frameshift mutation will also alter the first stop codon (""UAA"", ""UGA"" or ""UAG"") encountered in the sequence. The polypeptide being created could be abnormally short or abnormally long, and will most likely not be functional.Frameshift mutations are apparent in severe genetic diseases such as Tay-Sachs disease and Cystic Fibrosis; they increase susceptibility to certain cancers and classes of familial hypercholesterolaemia; in 1997, a frameshift mutation was linked to resistance to infection by the HIV retrovirus. Frameshift mutations have been proposed as a source of biological novelty, as with the alleged creation of nylonase, however, this interpretation is controversial. A study by Negoro et al (2006) found that a frameshift mutation was unlikely to have been the cause and that rather a two amino acid substitution in the catalytic cleft of an ancestral esterase amplified Ald-hydrolytic activity.
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