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promoting training adaptations through nutritional
promoting training adaptations through nutritional

... normal glycogen levels (Wojtasezewski et al. 2003). AMPK is also likely to mediate the contractioninduced increase in glucose uptake (Hayashi et al. 1998) and thus may play a role in promoting postexercise glycogen accumulation in skeletal muscle (Barnes et al. 2005; Carling and Hardie 1989; Sakoda ...
proteinstructure
proteinstructure

... Slide 10 of 56 ...
Feeney_ku_0099D_12934_DATA_1 - KU ScholarWorks
Feeney_ku_0099D_12934_DATA_1 - KU ScholarWorks

... ii ...
Reactive cysteine in proteins: Protein folding - Genoma
Reactive cysteine in proteins: Protein folding - Genoma

... CXXC motif (typically CGPC), which are used to reduce target proteins that are recognized by other domains of thioredoxin polypeptide. The reduction of target proteins results in a disulfide bridge between the two cysteines from the thioredoxin CXXC motif, which is then reduced by thioredoxin reduct ...
Peptide Formulation: Challenges and Strategies
Peptide Formulation: Challenges and Strategies

... landscape exists. Therefore, the aqueous solution conformation may have little similarity to the ‘active’ structure found when bound to a receptor. Either the receptor induces a conformational fit, or the peptide binds to a membrane and diffuses in two dimensions to the receptor, making the lipid-bo ...
PDF
PDF

... Methodology/Principal Findings. The whole genome sequence of the diatom Phaeodactylum tricornutum has recently been completed. We identified and annotated genes for enzymes involved in carbohydrate pathways based on extensive EST support and comparison to the whole genome sequence of a second diatom ...
Plant Physiology
Plant Physiology

... rice plants displayed higher starch content than wildtype plants grown under both well-watered and waterstress conditions (Fig. 2B; Peleg et al., 2011). Despite the increase in amylolytic activity found in wild-type plants with the stress, no significant differences were found in amylolytic activity ...
Supporting Information
Supporting Information

... (11-cis-retinol) was purified by HPLC (10% ethyl acetate in hexane at a flow rate of 1.4 mL/min; Agilent-Si; 5 ␮m, 4.5 ⫻ 250 mm). Because an intact retinol molecule is difficult to analyze by LC-MS because of the loss of water on ionization, we converted the retinol products to 13,14-dihydroretinol ...
Differential scanning calorimetry as tool in observing thermal and
Differential scanning calorimetry as tool in observing thermal and

... reviewed (Cueto et al., 2003; Gao et al., 2005). Protein structures are stabilized by non-covalent intramolecular interactions between amino acid side chains. The higher levels of protein structure are maintained by relatively weak non-covalent interactions. Disruption of these weak interactions can ...
Lecture 1 – Classification - LCQB
Lecture 1 – Classification - LCQB

... Structural motifs Structural motifs are simple combinations of a few secondary structure elements, with a specific geometrical arrangement. Some motifs may be associated to a particular function. ...
Structure, prediction, evolution and genome wide studies of membrane proteins
Structure, prediction, evolution and genome wide studies of membrane proteins

... waiting patiently for at least 10 billion years for this to happen and ever since that day, nothing has been quite the same. The cells replicated and replicated and finally a group of cells wrote this thesis. In order to have a functional, living cell some of the basic things that are needed are: • ...
Lecture 1 – Classification - LCQB
Lecture 1 – Classification - LCQB

... Structural motifs Structural motifs are simple combinations of a few secondary structure elements, with a specific geometrical arrangement. Some motifs may be associated to a particular function. ...
Properties of a newly characterized protein of the bovine - K-REx
Properties of a newly characterized protein of the bovine - K-REx

... with rapid reuse of a single lipoyl moiety. It is believed that the former may be more likely. In summary, the E2 subunit differs between mammalian and in molecular weight and quaternary structure. ...
Partial Purification and Characterization of Three Flavonol
Partial Purification and Characterization of Three Flavonol

... Three distinct flavonol-specific sulfotransferases were partially purified from the shoot tips of Flaveria chloraefolia A. Gray by fractional precipitation with ammonium sulfate, followed by gel filtration on Sephacryl S-200, 3'-phosphoadenosine 5-phosphateAgarose affinity chromatography and chromat ...
Safety Assessment of Soy Proteins and Peptides as Used in
Safety Assessment of Soy Proteins and Peptides as Used in

... (soybean). Both names refer to the same plant species.8 This change represents more commonly accepted nomenclature. CHEMISTRY Definition and Manufacture The definitions and functions of the soy peptide ingredients included in this report are provided in Table 1. The soy protein and peptide derivativ ...
Development of prokaryotic cell-free systems for synthetic
Development of prokaryotic cell-free systems for synthetic

... While strains can be specifically engineered for protein production or for specific applications, there is also a focus on using “generic” strains for cell-free prototyping. Reasons for using generic strains rather than specialized strains include: (1) Lack of need of high protein expression, and (2 ...
Cis-trans peptide variations in structurally similar proteins.
Cis-trans peptide variations in structurally similar proteins.

... types VIa1, VIa2 and VIb (that are associated with a cis-Pro (Hutchinson and Thornton 1996)) also dominate the cis conformation. The conformation at the first position (Figure 3A) is represented by β turns VIa1 and VIb with an occurrences of 13.1% and 16.2% respectively. The coil and extended (β str ...
Investigating semantic similarity measures across the
Investigating semantic similarity measures across the

... “granum”, (GO:0009542) becomes a kind of “cellular component”, (GO:0005575), rather than a kind of a “chloroplast component”), but this ontological sleight of hand made our semantic measurement possible. It is also unclear how we should address the different link types. Except where stated explicitl ...
Towards a Semantic W..
Towards a Semantic W..

... ortholog : Orthologs are genes in different species that have evolved from a common ancestral gene by speciation. Orthologs usually share the same functions in the course of evolution. ...
Structural And Functional Studies Of Nicotinamide Adenine
Structural And Functional Studies Of Nicotinamide Adenine

... Invasive infections caused by Streptococcus pyogenes, also known as Group A Strep (GAS), results in approximately 600,000 deaths annually. With evidence of antibiotic-resistant strains of this bacterium on the rise, there is a need for the identification of new drug targets to control these infectio ...
The Commercial Potential for Genetic Engineering in Alfalfa Mark
The Commercial Potential for Genetic Engineering in Alfalfa Mark

... etc. will greatly effect the expression level of the target gene, and dictate the required intellectual property (IP) licenses for commercialization. Transformation – alfalfa has been successfully transformed with both Agrobacterium vectors and with particle bombardment. In most cases Agrobacterium- ...
Regulation of metabolism by dietary carbohydrates in two lines of
Regulation of metabolism by dietary carbohydrates in two lines of

... (ACLY; EC 2.3.3.8), fatty acid synthase (FAS; EC 2.3.1.85) and the transcription factor sterol regulatory element binding protein 1like (SREBP-1c) for lipogenesis; 6 desaturase (D6D; EC 1.14.19.3) and 9 desaturase (D9D; EC 1.14.19.1) for fatty acid bioconversion; and carnitine palmitoyl transferas ...
Article, Discoveries Variation in mutational robustness between
Article, Discoveries Variation in mutational robustness between

... evolutionary relevance of such mutational landscapes is uncertain. For example, Kondrashov and co-workers (Sarkisyan et al. 2016) measured the effects of mutations in Green Fluorescent Protein (GFP). In this case, the gene was moved from its original organism, the jellyfish Aequorea victoria, into E ...
Ketone Bodies Mimic the Life Span Extending
Ketone Bodies Mimic the Life Span Extending

... The toxicity of ROS/RNS is ameliorated by the NADPH system (Fig. 2), the redox potential of which is made more negative by the metabolism of ketone bodies (19,38,39). The redox potential of the free cytosolic [NADP1]/[NADPH] system is about 20.42 V, about the same redox potential as hydrogen and is ...
Document
Document

... 12. Feeney, R.E., Whitaker, J.R., Protein Tailoring & Reagents for Food & Medical Uses, Marcel Dekker Incorporated, 1986, 0-82477616-X. 13. Bailey, J. L., Techniques in Protein Chemistry, Elsevier Publishing Company, 1962, Lib. Congress 62-19691. 14. Lundblad, R. L., Chemical Reagents for Protein Mo ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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