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video slide
video slide

... polypeptides coiled like a rope  Hemoglobin is a globular protein consisting of four polypeptides: two alpha and two beta ...
Proteins Quiz - cloudfront.net
Proteins Quiz - cloudfront.net

... Proteins Quiz 1. Roughly how many amino acids are present in a polypeptide? a) 5-10 b) 10-100 c) 30-60 d) more than 80 ...
1 Supplementary data Materials and methods Preparation of the
1 Supplementary data Materials and methods Preparation of the

... with a rotating anode (RU-300, Rigaku) and an R-AXIS IV detector (Molecular Structure Corporation). Diffraction data for ADP- and ATP-bound complexes were collected with beamlines 19ID and 19BM in the Structural Biology Center at the Advanced Photon Source (Argonne, IL). The data were processed wit ...
Proteins - RHS AP Biology
Proteins - RHS AP Biology

... A protein is simply a long chain of amino acids linked together by bonds. The backbone of amino acids form strong covalent bonds and the actual amino acids form temporary weak bonds. These weak bonds allow the amino acids to change shape, remain mobile, and attain flexibility. The most important qua ...
chapter 3 - rci.rutgers.edu
chapter 3 - rci.rutgers.edu

... Primary structure of a protein is the linear sequence of amino acids in the protein. This is unique for each protein, and comes from sequences stored in the DNA genes. Secondary structure is repeated small-scale structure. The two most important secondary structures, the -helix and the -sheet, are ...
Leukaemia Section del(11)(q23q23) MLL/ARHGEF12 Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section del(11)(q23q23) MLL/ARHGEF12 Atlas of Genetics and Cytogenetics in Oncology and Haematology

... domain: methyltransferase; methylates H3, including histones in the HOX area for allowing chromatin to be open to transcription. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus: a p300/320 Nterm protein called MLL-N, and a p180 C-term protein, called MLL-C. The FYRN and a FRY ...
Virus production in packaging cell lines
Virus production in packaging cell lines

... gene of interest can be cloned into the multiple cloning site downstream of the CMV promoter. Plasmid includes the Col E1 Ori and Ampr gene for propagation and antibiotic selection in bacteria. ...
Human EGF / Epidermal Growth Factor Protein
Human EGF / Epidermal Growth Factor Protein

... integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of bioche ...
GENETIC ENGINEERING AND BIOTECHNOLOGY Course code
GENETIC ENGINEERING AND BIOTECHNOLOGY Course code

... biotechnology. It then examines the molecular cloning methods, the various cloning vectors and their hosts, and how to find the right vector for molecular cloning. Aspects of DNA amplification and analysis techniques, cloning and expression of mammalian and plant genes in bacteria and practical appl ...
No Slide Title
No Slide Title

... Uretupamine A & B SpecificityA Whole-Genome Gene Expression Profiling - A subset of genes known to be repressed by Ure2p where up regulated - These subset of genes was not changed in expression when a Ure2p deletion strain was assayed with Uretupamine A & B ...
Protein Structure:
Protein Structure:

... functions in essentially all biological processes. They function as catalysts, they transport and store other molecules such as oxygen, they provide mechanical support and immune protection, they generate movement, they transmit nerve impulses, and they control growth and differentiation. Indeed, mu ...
A European Infrastructure of Ligand Binding
A European Infrastructure of Ligand Binding

... quality control for these essential reagents. We aim to provide a set of consistently  characterised binders, required to detect all the relevant human proteins in tissues  and fluids in health and disease. As the size of the human proteome is at least an  order of magnitude greater than the ~ 21.00 ...
PROTEOME:
PROTEOME:

... analyzer in series with a collision cell in between • Collision cell: a region where the ions collide with a gas (He, Ne, Ar) resulting in fragmentation of the ion • Fragmentation of the peptides in the collision cell occur in a predictable fashion, mainly at the peptide bonds (also ...
Biochemistry Chapter 17
Biochemistry Chapter 17

... contain almost every vitamin and mineral you need. The egg white or albumen contains lots of protein (ovalbumin) and not much else. The egg yolk contains all of the fat and most of the other nutrients found in eggs. It is also high in protein (livetin). ...
Plant transformation methods
Plant transformation methods

... a new source of biofuels since it produces a number of diesel compounds from cellulose. "This is the only organism that has ever been shown to produce such an important combination of fuel substances," said Montana State University Professor Gary Strobel, making it a better source of biofuels than a ...
LECT09 fibro
LECT09 fibro

... bond covalently to either N or O is attracted by an electron pair from a neighboring N or O. The attracting force is basically electrostatic. Disulfide Bond: A strong covalent bond formed by two –SH groups of cysteines. This bond can only be broken to component -SH groups by reducing agents. Electro ...
Hypertension-Associated Transcription Factor (HATF-1)
Hypertension-Associated Transcription Factor (HATF-1)

... expressed in SHR brains as early as 3 days post-partum. Northern blot analysis over a developmental timecourse from 3 to 10 days post-partum indicates a two to four fold increase in mRNA levels in SHR brains compared to the normotensive controls. This transcript, which we have named Hypertension-ass ...
protein - 4J Blog Server
protein - 4J Blog Server

... the denaturing impact that heat and pH can have on protein structure, and how these may affect the organism. • The directionality of proteins (the amino and carboxyl ends). ...
What is a protein?
What is a protein?

... (hydrogen bonds) 3° = 3D structure ...
Post-translational modifications on human cell expressed
Post-translational modifications on human cell expressed

... the sites of glycosylation. Removing the Nlinked oligosaccharides using enzymatic methods changes the asparagine amino acid to an aspartic acid. This changes the mass of the peptide, hence enabling the determination of whether the site is non-, ...
Lecture 22: Protein Engineering
Lecture 22: Protein Engineering

... used to generate randomized genomic libraries. It allows the initiation of DNA amplification, starting with tiny amounts of parent molecule, and produces considerable amounts of the mutated gene. The working principle of this technique is based on the ability of Taq polymerase to anneal incompatible ...
Gene Section MAPK4 (mitogen-activated protein kinase 4) Atlas of Genetics and Cytogenetics
Gene Section MAPK4 (mitogen-activated protein kinase 4) Atlas of Genetics and Cytogenetics

... amino acids and contains a typical kinase domain located at the N-terminal extremity. Another region with homology to the MAP kinase ERK3 (C34 domain) has been identified after the kinase domain. The function of the C34 domain is unknown. ...
Materials and methods (Supplement)
Materials and methods (Supplement)

... mechanism (Fig. 2D, type I) [17] (Supplement Fig. 2A). However, additional short sequences were present in the footprint sequences of the plasmid DNA after excision. These additional sequences can be categorized as two types. Type II has short direct repeats, as exemplified by clone 2b, as well as b ...
LEA proteins in higher plants: Structure, function, gene expression
LEA proteins in higher plants: Structure, function, gene expression

... and analyze stress responses of crops. The extensive application of transgenic plants and microarrays is one developing direction. The related evidence directly from transgenic plants to support anti-drought functions of LEA proteins is not so much. By utilizing an individual-function gene, the obta ...
Nanotechnology in Medicine Krešimir Pavelić Division of Molecular
Nanotechnology in Medicine Krešimir Pavelić Division of Molecular

... nanoparticles, has been considered. (The size of nanocapsules - 350 + 100 nm). ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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