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Test Results - Oregon State University
Test Results - Oregon State University

... Quiz study partner Discuss subjects with friends Grill your T.A. at recitation about the subject matter ...
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Protein Structure & Function - Lectures For UG-5
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... sites was compared to that published by Brugère et al.4. Assignment of contigs to chromosomes was based on known genetic markers5 and/or by hybridization of Southern blots of E. cuniculi chromosomes separated by pulsed-field gel electrophoresis. Blots were hybridized using standard procedures with s ...
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Protein Analysis

... – This residue cyclizes under acidic conditions to give a PTH amino acid and a peptide shortened by one amino acid residue. – This PTH-amino acid is identified by HPLC. – Automated repeated Edman degradation by a sequenator that can analyze sequences of about 50 amino acids long. – The amino acid co ...
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No Slide Title

... homology based on sequence identity may be subtle. Consider RBP and OBP: these are true homologs (they are both lipocalins, sharing the GXW motif). But they are distant relatives, and do not share significant amino acid identity in a pairwise alignment. ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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