High efficiency, site-specific excision of a marker gene by the phage
... This cloning results in a plasmid with the site-speci®c recognition sequences inserted into a multiple cloning site (polylinker). In a separate reaction, pBSMos1, containing the mariner transposable element, Mos1 (18,19), was digested with SacI and the vector backbone fragment containing the Mos1 ri ...
... This cloning results in a plasmid with the site-speci®c recognition sequences inserted into a multiple cloning site (polylinker). In a separate reaction, pBSMos1, containing the mariner transposable element, Mos1 (18,19), was digested with SacI and the vector backbone fragment containing the Mos1 ri ...
Laboratory of Insect Genetics and Biosciences (IGB) Dept. Biology
... the DNA of mammalian somatic cells”. A recent paper showed the existence of non-CG methylation in mammalian somatic cells (PMID:26030523). In fact, non-CG methylation in mammals seems to be more informative of gene expression than CG methylation. R: It is true that DNA methylation has been detected ...
... the DNA of mammalian somatic cells”. A recent paper showed the existence of non-CG methylation in mammalian somatic cells (PMID:26030523). In fact, non-CG methylation in mammals seems to be more informative of gene expression than CG methylation. R: It is true that DNA methylation has been detected ...
Cloning: A Critical Analysis of Myths and Media
... also used in genetic engineering efforts, such as transgenic technology (where DNA from one species is inserted into another) and/or gene pharming (where scientists alter an animal’s DNA so that it can make human proteins, drugs, or compounds that have medicinal or other applications). Transgenic an ...
... also used in genetic engineering efforts, such as transgenic technology (where DNA from one species is inserted into another) and/or gene pharming (where scientists alter an animal’s DNA so that it can make human proteins, drugs, or compounds that have medicinal or other applications). Transgenic an ...
Document
... Carefully remove the supernatant and wash the pellet with 500 ul of chilled 70% ethanol. You want the pellet to break free of the wall and break apart, but the debris doesn’t have to completely disappear. 15. Centrifuge for 5 minutes at 4 C. After removing supernatant, sit the tube upside down on a ...
... Carefully remove the supernatant and wash the pellet with 500 ul of chilled 70% ethanol. You want the pellet to break free of the wall and break apart, but the debris doesn’t have to completely disappear. 15. Centrifuge for 5 minutes at 4 C. After removing supernatant, sit the tube upside down on a ...
Registration of facility
... Access, Expression and Damage, which together with an overall indication of which level of containment should be used. 9.1 Risk of Access Assess the probability that a microorganism, or the DNA contained within it, will be able to enter the human body and survive there, or survive in the environment ...
... Access, Expression and Damage, which together with an overall indication of which level of containment should be used. 9.1 Risk of Access Assess the probability that a microorganism, or the DNA contained within it, will be able to enter the human body and survive there, or survive in the environment ...
Agrobacterium-mediated DNA transfer, and then some
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
AUTOMATED DNA SEQUENCING, MegaBACE 1000
... Because the slab gel electrophoresis was still manual and needed several steps, modern capillary systems were introduced. These systems are currently the fastest system available they use the same basic electrophoresis and detection method technology as the slab ...
... Because the slab gel electrophoresis was still manual and needed several steps, modern capillary systems were introduced. These systems are currently the fastest system available they use the same basic electrophoresis and detection method technology as the slab ...
DNA Lesson 2 Guide
... 2. Build the DNA on top of the gene strip. Check that your nucleotides match the strip exactly. The bottom strand of DNA is the gene. 3. Notice the DNA nucleotides on the bottom of your gene strip are marked in groups of 3 with dark gray boxes. A group of 3 nucleotides is called a codon. ...
... 2. Build the DNA on top of the gene strip. Check that your nucleotides match the strip exactly. The bottom strand of DNA is the gene. 3. Notice the DNA nucleotides on the bottom of your gene strip are marked in groups of 3 with dark gray boxes. A group of 3 nucleotides is called a codon. ...
GPR17 shRNA Plasmid (r): sc-270023-SH
... GPR17 shRNA Plasmid (r) is a target-specific lentiviral vector plasmid encoding a 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 20 µg of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also see GPR17 siRNA (r): sc-270023 and GPR17 shRNA ...
... GPR17 shRNA Plasmid (r) is a target-specific lentiviral vector plasmid encoding a 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 20 µg of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also see GPR17 siRNA (r): sc-270023 and GPR17 shRNA ...
- DigitalCommons@Linfield
... transcripGon factor is the thyroid hormone receptor (Psarra & Sekeris, 2008). Previous research suggests that when the hormone triiodothyronine (T3) is present and taken up in cells, mitochondrial ...
... transcripGon factor is the thyroid hormone receptor (Psarra & Sekeris, 2008). Previous research suggests that when the hormone triiodothyronine (T3) is present and taken up in cells, mitochondrial ...
ES5
... Sequencing and “Barcode Analysis” of Microbial Communities PCR products from Bacteria and Fungal reactions are in cue to be sequenced on an Illumina MiSEQ platform. The results from this technology are analogous to data derived from cloning and sequencing in that single-stranded reads are generated ...
... Sequencing and “Barcode Analysis” of Microbial Communities PCR products from Bacteria and Fungal reactions are in cue to be sequenced on an Illumina MiSEQ platform. The results from this technology are analogous to data derived from cloning and sequencing in that single-stranded reads are generated ...
High Throughput Screening of Single Nucleotide Polymorphisms
... similar to those obtained in dideoxy sequencing reactions using a brief (15-30 minutes) enzymatic treatment of the PCR product. The BESS reaction products can be analyzed using a capillary or gel just like dideoxy sequencing reactions. The mechanisms of BESS reactions are presented below. ...
... similar to those obtained in dideoxy sequencing reactions using a brief (15-30 minutes) enzymatic treatment of the PCR product. The BESS reaction products can be analyzed using a capillary or gel just like dideoxy sequencing reactions. The mechanisms of BESS reactions are presented below. ...
Reviewing Biology: The Living Environment
... information that is expressed as the traits of the organism, a phenomenon called gene expression. A single gene (that is, one set of alleles) may control one or several traits. Alternatively, some traits are determined by more than one gene (that is, by more than one set of alleles). Although all th ...
... information that is expressed as the traits of the organism, a phenomenon called gene expression. A single gene (that is, one set of alleles) may control one or several traits. Alternatively, some traits are determined by more than one gene (that is, by more than one set of alleles). Although all th ...
1 - marric
... removed three nucleotides. The bacterium appears completely unaffected in all its functions. Where is the mostly likely location for the mutation? Introns, or intervening sequences, which get processed out of the mRNA before it leaves the nucleus, so removal of an intron would probably have little e ...
... removed three nucleotides. The bacterium appears completely unaffected in all its functions. Where is the mostly likely location for the mutation? Introns, or intervening sequences, which get processed out of the mRNA before it leaves the nucleus, so removal of an intron would probably have little e ...
Chemistry Problem Solving Drill
... (A) Chemical, tissue, cell, organ, system, organism. (B) Cell, chemical, tissue, organ, system, organism. (C) Tissue, cell, chemical, organ, organism, system. (D) Chemical, cell, tissue, organ, system, organism. A. Incorrect! Tissues are collections of cells and surrounding matrix. B. Incorrect! Cel ...
... (A) Chemical, tissue, cell, organ, system, organism. (B) Cell, chemical, tissue, organ, system, organism. (C) Tissue, cell, chemical, organ, organism, system. (D) Chemical, cell, tissue, organ, system, organism. A. Incorrect! Tissues are collections of cells and surrounding matrix. B. Incorrect! Cel ...
IBC Declaration
... a dealing involving the introduction of a replication defective viral vector unable to transduce human cells into a host not mentioned in Part 2 of Schedule 2, if the donor nucleic acid cannot restore replication competence to the vector; a dealing involving the introduction of a replication defecti ...
... a dealing involving the introduction of a replication defective viral vector unable to transduce human cells into a host not mentioned in Part 2 of Schedule 2, if the donor nucleic acid cannot restore replication competence to the vector; a dealing involving the introduction of a replication defecti ...
Chapter 11 Transcription and RNA Processing
... the DNA template (antisense) strand and identical (except that uridine replaces thymidine) to the DNA nontemplate (sense) strand. RNA synthesis is catalyzed by RNA polymerases and proceeds in the 5’3’ direction. ...
... the DNA template (antisense) strand and identical (except that uridine replaces thymidine) to the DNA nontemplate (sense) strand. RNA synthesis is catalyzed by RNA polymerases and proceeds in the 5’3’ direction. ...
Reactive Oxygen Species
... A scheme of the catalytic cycle of cytochrome P450-containing monooxygenases. The binding of the substrate (RH) to ferric P450 (a) results in the formation of the substrate complex (b). The ferric P450 then accepts the first electron from CPR (cytochrome P450 reductase), thereby being reduced to the ...
... A scheme of the catalytic cycle of cytochrome P450-containing monooxygenases. The binding of the substrate (RH) to ferric P450 (a) results in the formation of the substrate complex (b). The ferric P450 then accepts the first electron from CPR (cytochrome P450 reductase), thereby being reduced to the ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.