Microsoft Word - Mapping-Traits-in-Dogs
... Ostrander, Ph.D., chief of the Cancer Genetics Branch in NHGRI's Division of Intramural Research. "We think this approach will help pinpoint multiple genes involved in complex human conditions, such as cancer, heart disease, diabetes and obesity." Artificial selection, at the heart of breeding for d ...
... Ostrander, Ph.D., chief of the Cancer Genetics Branch in NHGRI's Division of Intramural Research. "We think this approach will help pinpoint multiple genes involved in complex human conditions, such as cancer, heart disease, diabetes and obesity." Artificial selection, at the heart of breeding for d ...
disease revealed by a metagenomic approach Reduced diversity of
... inserts having similar end sequences (data not shown), confirmed that major biases can be avoided by using this method. The DNA macroarrays were constructed after fosmid DNA extraction from the 50 000 recombinant clones and spotting on two nylon membranes, one for HSL and one for CPL (fig 1). 16S rR ...
... inserts having similar end sequences (data not shown), confirmed that major biases can be avoided by using this method. The DNA macroarrays were constructed after fosmid DNA extraction from the 50 000 recombinant clones and spotting on two nylon membranes, one for HSL and one for CPL (fig 1). 16S rR ...
Isolation and characterization of cDNA clones encoding
... class were further characterized by DNA sequence analysis. All clones analyzed contained the 138 amino acid coding regions of their respective mature proteins, but only partial sequences of the signal peptides. Minor differences between the nucleotide sequences for clones belonging to the same class ...
... class were further characterized by DNA sequence analysis. All clones analyzed contained the 138 amino acid coding regions of their respective mature proteins, but only partial sequences of the signal peptides. Minor differences between the nucleotide sequences for clones belonging to the same class ...
Sathgudi Sweet orange
... 2. The complete genome of CMBVSON consisted of 7558 nucleotide containing for 6 open reading frames (ORFs). High variability (87.4% to 96%) observed in ORF 4 and 5 infecting sathgudi sweet orange, rangpur lime acid lime and pummelo. 3. The intergenic region (non coding region) has 731 nucleotide. In ...
... 2. The complete genome of CMBVSON consisted of 7558 nucleotide containing for 6 open reading frames (ORFs). High variability (87.4% to 96%) observed in ORF 4 and 5 infecting sathgudi sweet orange, rangpur lime acid lime and pummelo. 3. The intergenic region (non coding region) has 731 nucleotide. In ...
Brassica genome structure
... •Amphidiploid: diploid set of chromosomes from each parent •N=19 •Multiple fusion events between diploid B. rapa and B. oleracea •A genome, n=10, B. rapa •B genome, n=8, B. nigra •C genome, n=9, B. oleracea ...
... •Amphidiploid: diploid set of chromosomes from each parent •N=19 •Multiple fusion events between diploid B. rapa and B. oleracea •A genome, n=10, B. rapa •B genome, n=8, B. nigra •C genome, n=9, B. oleracea ...
A phage library and two cosmid libraries were
... human placenta DNA were used. The insert of the M13 subclone 1-1 was used to screen both cosmid libraries. A total number of 27 recombinant cosmid clones were isolated and analysed by detailed restriction mapping. Only 15 of these clones yielded a positive hybridization signal with the smaller V,, g ...
... human placenta DNA were used. The insert of the M13 subclone 1-1 was used to screen both cosmid libraries. A total number of 27 recombinant cosmid clones were isolated and analysed by detailed restriction mapping. Only 15 of these clones yielded a positive hybridization signal with the smaller V,, g ...
Online resources for genetic variation study-Part One
... If most SNPs have highly significant correlation to one or more of neighbors, these correlations can be used to generate haplotypes, which represent excellent proxies for individual SNP. Because haplotypes may be identified by a much small number of SNPs (tag SNPs), assessing polymorphisms via h ...
... If most SNPs have highly significant correlation to one or more of neighbors, these correlations can be used to generate haplotypes, which represent excellent proxies for individual SNP. Because haplotypes may be identified by a much small number of SNPs (tag SNPs), assessing polymorphisms via h ...
Predicting Cancer Susceptibility from Single-Nucleotide
... In its simplest form, a support vector machine is an algorithm that attempts to find a linear separator between the data points of two classes, as Figure 2 illustrates. SVMs seek to maximize the margin, or the separation between the two classes, in order to improve the chance of making accurate pred ...
... In its simplest form, a support vector machine is an algorithm that attempts to find a linear separator between the data points of two classes, as Figure 2 illustrates. SVMs seek to maximize the margin, or the separation between the two classes, in order to improve the chance of making accurate pred ...
Supplementary Information
... Table S2. GISTIC significant events and resident genes with copy number-correlated expression. Table S3. Significant alterations identified by RAE including resident genes with correlated expression. Table S4. GTS defined regions of informative CNAs. Table S5. Phase I genes (n=601) selected for re-s ...
... Table S2. GISTIC significant events and resident genes with copy number-correlated expression. Table S3. Significant alterations identified by RAE including resident genes with correlated expression. Table S4. GTS defined regions of informative CNAs. Table S5. Phase I genes (n=601) selected for re-s ...
Detecting HFE Mutations in Human Genomic DNA
... The purpose of this laboratory is to screen human genomic DNA samples for mutations in the HFE gene that are linked to hereditary hemochromatosis. Students will use PCR to amplify exons 2 and 4 from the HFE gene and look for restriction fragment length polymorphisms associated with the mutations. Th ...
... The purpose of this laboratory is to screen human genomic DNA samples for mutations in the HFE gene that are linked to hereditary hemochromatosis. Students will use PCR to amplify exons 2 and 4 from the HFE gene and look for restriction fragment length polymorphisms associated with the mutations. Th ...
Supplementary Information to manuscript Microbial life in the Lake
... For aerobic-microaerophilic incubations (deep WC and TZ), 40 mL for each sample in triplicate and one formaldehyde-fixed blank control were used. The determination of CO2 dark fixation rates for the micro-aerobic and anoxic samples (upper- and middle-interfaces and body brine) was performed into ser ...
... For aerobic-microaerophilic incubations (deep WC and TZ), 40 mL for each sample in triplicate and one formaldehyde-fixed blank control were used. The determination of CO2 dark fixation rates for the micro-aerobic and anoxic samples (upper- and middle-interfaces and body brine) was performed into ser ...
PDF
... yet reported in domestic animals. In humans it is rarely diagnosed and a majority of clinical features resemble those which are typical for Klinefelter syndrome (KS). Here we describe the first case of SRY-positive XX DSD in a tortoiseshell cat with a rudimentary penis and a lack of scrotum. Results ...
... yet reported in domestic animals. In humans it is rarely diagnosed and a majority of clinical features resemble those which are typical for Klinefelter syndrome (KS). Here we describe the first case of SRY-positive XX DSD in a tortoiseshell cat with a rudimentary penis and a lack of scrotum. Results ...
Full Paper - Biotechniques.org
... when this sample was taken, in aquatic systems at similar latitudes to that of Peters Kill (McMillan and Verduin 1953, Graham et al. 1985). Although both aliquots had the same genotype richness and dominant genotype, the community compositions of the two aliquots were significantly different. This d ...
... when this sample was taken, in aquatic systems at similar latitudes to that of Peters Kill (McMillan and Verduin 1953, Graham et al. 1985). Although both aliquots had the same genotype richness and dominant genotype, the community compositions of the two aliquots were significantly different. This d ...
Embryo Genome Profiling by Single-Cell
... whole-genome amplification (WGA) to amplify the biopsied embryonic single cell, the complete embryonic genome can be obtained to facilitate the diagnosis of genetic variants in embryos. However, the allele dropout (ADO) and preferential amplification (PA) in WGA still restrict the diagnostic accurac ...
... whole-genome amplification (WGA) to amplify the biopsied embryonic single cell, the complete embryonic genome can be obtained to facilitate the diagnosis of genetic variants in embryos. However, the allele dropout (ADO) and preferential amplification (PA) in WGA still restrict the diagnostic accurac ...
Chromosome mapping of the sweet potato little leaf
... phytoplasmas, a physical and genetic map of the sweet potato little leaf (SPLL) strain V4 phytoplasma chromosome was determined. PFGE was used to determine the size of the SPLL-V4 genome, which was estimated to be 622 kb. A physical map was prepared by two-dimensional reciprocal digestions using the ...
... phytoplasmas, a physical and genetic map of the sweet potato little leaf (SPLL) strain V4 phytoplasma chromosome was determined. PFGE was used to determine the size of the SPLL-V4 genome, which was estimated to be 622 kb. A physical map was prepared by two-dimensional reciprocal digestions using the ...
2013 Holiday Lectures on Science Medicine in the Genomic Era
... work together, rather than the genes themselves," said Elaine A. Ostrander, Ph.D., chief of the Cancer Genetics Branch in NHGRI's Division of Intramural Research. "We think this approach will help pinpoint ...
... work together, rather than the genes themselves," said Elaine A. Ostrander, Ph.D., chief of the Cancer Genetics Branch in NHGRI's Division of Intramural Research. "We think this approach will help pinpoint ...
A set reduction and pattern matching problem motivated by Allele
... the pool of sequences produced using the locus specific primer (Fig. 3). The three steps seem quite similar due to their common purpose: reduction of the existing set. However, they are distinct as the primers must be designed to leverage different regions and levels of conservation within the seque ...
... the pool of sequences produced using the locus specific primer (Fig. 3). The three steps seem quite similar due to their common purpose: reduction of the existing set. However, they are distinct as the primers must be designed to leverage different regions and levels of conservation within the seque ...
Open Access - Cambridge Neuroscience
... Funding: This work was funded by grants to SBC from the Nancy Lurie Marks Family Foundation, the Medical Research Council (MRC) UK, and the Autism Research Trust (ART). LM and SEF were supported by the Max Planck Society. The funders had no role in study design, data collection and analysis, decisio ...
... Funding: This work was funded by grants to SBC from the Nancy Lurie Marks Family Foundation, the Medical Research Council (MRC) UK, and the Autism Research Trust (ART). LM and SEF were supported by the Max Planck Society. The funders had no role in study design, data collection and analysis, decisio ...
Avian Infectious Bronchitis Virus (IBV)
... amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The ...
... amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The ...
Cytogenetic and AZF microdeletions on the Y chromosome of
... Deletion testing was performed in men with a sperm concentration <5.11/mL (azoospermia or sever oligozoospermia). Extraction of genomic DNA from peripheral blood lymphocytes was performed using a standard technique. Sequence-Tagged Sites and Polymerase Chain Reaction The screening method for microde ...
... Deletion testing was performed in men with a sperm concentration <5.11/mL (azoospermia or sever oligozoospermia). Extraction of genomic DNA from peripheral blood lymphocytes was performed using a standard technique. Sequence-Tagged Sites and Polymerase Chain Reaction The screening method for microde ...
EPICENTRE Revolutionizes Cloning by Introducing CopyControl
... CopyControl Systems combine the clone stability afforded by single copy cloning with the advantages of high yields of DNA obtained by high copy vectors. The benefits of on-demand induction to obtain higher DNA yields from smaller amounts of bacterial culture will be obvious to genomics researchers u ...
... CopyControl Systems combine the clone stability afforded by single copy cloning with the advantages of high yields of DNA obtained by high copy vectors. The benefits of on-demand induction to obtain higher DNA yields from smaller amounts of bacterial culture will be obvious to genomics researchers u ...
Document
... Aldehyde oxidase (AO) is a molybdenum cofactor containing enzyme localized in the cytosol with relatively high expression levels in human hepatocytes. It is of increasing importance for drug development with an increasing number of new chemical entities (NCE) found to be substrates. Hepatic expressi ...
... Aldehyde oxidase (AO) is a molybdenum cofactor containing enzyme localized in the cytosol with relatively high expression levels in human hepatocytes. It is of increasing importance for drug development with an increasing number of new chemical entities (NCE) found to be substrates. Hepatic expressi ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.