Cot-1 banding of human chromosomes using fluorescence
... as an effective reagent to enhance specific binding of DNA probes to human genomic DNA immobilized on membrane filters. More recently, Cot-1 DNA is used for in situ hybridization to suppress non-specific binding of genomic DNA probes to chromosome spreads (Lichter et al., 1988, 1990a, 1990b). Partic ...
... as an effective reagent to enhance specific binding of DNA probes to human genomic DNA immobilized on membrane filters. More recently, Cot-1 DNA is used for in situ hybridization to suppress non-specific binding of genomic DNA probes to chromosome spreads (Lichter et al., 1988, 1990a, 1990b). Partic ...
Cloning, DNA nucleotide sequence and distribution
... pellet was redissolved in 4 pl distilled water. Sequencing reactions were done in accordance with the manufacturer’s recommendations. Primers for sequencing were synthesized on an ABI PCR-mate following the maker’s recommendations and were designed to give overlapping readings on both strands. The n ...
... pellet was redissolved in 4 pl distilled water. Sequencing reactions were done in accordance with the manufacturer’s recommendations. Primers for sequencing were synthesized on an ABI PCR-mate following the maker’s recommendations and were designed to give overlapping readings on both strands. The n ...
Cloning of Plastid Acetyl-CoA Carboxylase cDNA from Setaria italica
... from plastid and cytosol, which have Leu and Ile respectively at the herbicide binding site. The result showed that their molecular masses, native conformations and Michaelis constants for three substrates were all rather similar. Moreover, the reaction characteristics were close to an ordered mecha ...
... from plastid and cytosol, which have Leu and Ile respectively at the herbicide binding site. The result showed that their molecular masses, native conformations and Michaelis constants for three substrates were all rather similar. Moreover, the reaction characteristics were close to an ordered mecha ...
How the Genetics Calculator Creates Phenotype Names
... • Chromosome – Chromosomes occur in pairs, one inherited from each parent. Chromosomes contain the genetic information for each individual. • DNA – DNA is a long coiled molecule that makes up a chromosome. • Gene – A segment of DNA that codes for a specific trait. • Locus – Location on a chromosome ...
... • Chromosome – Chromosomes occur in pairs, one inherited from each parent. Chromosomes contain the genetic information for each individual. • DNA – DNA is a long coiled molecule that makes up a chromosome. • Gene – A segment of DNA that codes for a specific trait. • Locus – Location on a chromosome ...
Significance of bacterial identification by molecular
... enzyme to make a huge number of copies of virtually any given piece of DNA or gene. It facilitates a short stretch of DNA (usually fewer than 3000 bp) to be amplified by about a million-fold. In practical terms it amplifies enough specific copies to be able to carry out any number of other molecular ...
... enzyme to make a huge number of copies of virtually any given piece of DNA or gene. It facilitates a short stretch of DNA (usually fewer than 3000 bp) to be amplified by about a million-fold. In practical terms it amplifies enough specific copies to be able to carry out any number of other molecular ...
Supplementary Infomation (doc 1650K)
... Figure S2. Study-specific and meta-analysis quantile-quantile plots. Quantile–quantile plots and lambda (λ) values were estimated for the primary analysis using the Cochran–Armitage trend test and for the confirmatory analyzes using logistic regressions and Cochran–Mantel– Haenszel stratified tests. ...
... Figure S2. Study-specific and meta-analysis quantile-quantile plots. Quantile–quantile plots and lambda (λ) values were estimated for the primary analysis using the Cochran–Armitage trend test and for the confirmatory analyzes using logistic regressions and Cochran–Mantel– Haenszel stratified tests. ...
The first true obligately syntrophic propionate
... produced. Acetate was detected in only small amounts, indicating the presence of acetoclastic methanogens. Propionate-oxidizing bacteria were further purified by repeated rounds of pasteurization for 30 min at 85 uC and subculturing in the presence of Methanospirillum hungatei JF-1T. The highest dil ...
... produced. Acetate was detected in only small amounts, indicating the presence of acetoclastic methanogens. Propionate-oxidizing bacteria were further purified by repeated rounds of pasteurization for 30 min at 85 uC and subculturing in the presence of Methanospirillum hungatei JF-1T. The highest dil ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... regions are ideal candidates to determine differential drug responses [18]. A number of important SNPs have also been identified within critical gene promoters, which are noncoding regions [19]. The differential drug metabolism caused by genetic variation for the CYP enzymes and in the μ opioid-rece ...
... regions are ideal candidates to determine differential drug responses [18]. A number of important SNPs have also been identified within critical gene promoters, which are noncoding regions [19]. The differential drug metabolism caused by genetic variation for the CYP enzymes and in the μ opioid-rece ...
Justification of Size Estimates for Tomato Genome Sequencing
... Additional Information. When the sequencing project is advanced to the stage where BAC contigs can be assayed for both total non-redundant sequence length and physical distance based on in situ hybridization, we will be able to develop an additional estimate of euchromatin physical size through vali ...
... Additional Information. When the sequencing project is advanced to the stage where BAC contigs can be assayed for both total non-redundant sequence length and physical distance based on in situ hybridization, we will be able to develop an additional estimate of euchromatin physical size through vali ...
Marker-assisted backcross breeding
... Genotyping by sequencing (GBS) RAD-seq and RNA-seq SNP discovery and validation QTL mapping and association analysis Candidate gene identified and clone ...
... Genotyping by sequencing (GBS) RAD-seq and RNA-seq SNP discovery and validation QTL mapping and association analysis Candidate gene identified and clone ...
Identification of a Cis-Acting Element of ART1, a
... nucleotides (GG) are critical for ART1 binding (Fig. 4B). By contrast, substitution of T to C (probe PM9) at the position of 2341 did not affect the binding to ART1 protein although substitution to G and A (probes PM7 and 8) at the same position resulted in weakened signal. Substitution of C to A an ...
... nucleotides (GG) are critical for ART1 binding (Fig. 4B). By contrast, substitution of T to C (probe PM9) at the position of 2341 did not affect the binding to ART1 protein although substitution to G and A (probes PM7 and 8) at the same position resulted in weakened signal. Substitution of C to A an ...
Influence of the environment and probes on rapid DNA sequencing
... overlapping. This means that a handful of measurements of a base to be sequenced would not be enough to distinguish it from the other bases. However, in a real experiment each measurement would take a finite amount of time to be performed (finite inverse bandwidth indicated with ∆t in Figure 2b). In ...
... overlapping. This means that a handful of measurements of a base to be sequenced would not be enough to distinguish it from the other bases. However, in a real experiment each measurement would take a finite amount of time to be performed (finite inverse bandwidth indicated with ∆t in Figure 2b). In ...
The Non-LTR Retrotransposon Rex3 from the Fish Xiphophorus is
... 1999). To our knowledge, an RT-carrying retrotransposon has not previously been reported as being widespread in bony fishes or even in teleosts. Fish of the genus Xiphophorus (Poeciliidae) are inhabitants of freshwater biotopes of the Atlantic drainage of Mexico, Honduras, and Guatemala. Formation o ...
... 1999). To our knowledge, an RT-carrying retrotransposon has not previously been reported as being widespread in bony fishes or even in teleosts. Fish of the genus Xiphophorus (Poeciliidae) are inhabitants of freshwater biotopes of the Atlantic drainage of Mexico, Honduras, and Guatemala. Formation o ...
Using a Single Nucleotide Polymorphism to Predict Bitter
... repeat is a short sequence of DNA that is repeated in a headto-tail fashion at a specific chromosomal locus. Tandem repeats are interspersed throughout the human genome. Some sequences are found at only one site -- a single locus - in the human genome. For many tandem repeats, the number of repeated ...
... repeat is a short sequence of DNA that is repeated in a headto-tail fashion at a specific chromosomal locus. Tandem repeats are interspersed throughout the human genome. Some sequences are found at only one site -- a single locus - in the human genome. For many tandem repeats, the number of repeated ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
Y-Chromosome Marker S28 / U152 Haplogroup
... deposited in the online dbSNP database. Gareth Henson noticed three SNPs that appeared to be associated with M269, what was then known as haplogroup R1b1c. Dr. James F. Wilson of EthnoAncestry developed primers for these Single Nucleotide Polymorphism (SNP) markers on the Y-chromosome, one of which ...
... deposited in the online dbSNP database. Gareth Henson noticed three SNPs that appeared to be associated with M269, what was then known as haplogroup R1b1c. Dr. James F. Wilson of EthnoAncestry developed primers for these Single Nucleotide Polymorphism (SNP) markers on the Y-chromosome, one of which ...
In silico method for inferring genotypes in pedigrees
... Gene mapping projects often begin with a linkage study with relatively sparse markers. When candidate regions are found, they are further investigated by association analysis. Because association studies require a dense set of markers, the cost of conventional genotyping can be very high. Here, we s ...
... Gene mapping projects often begin with a linkage study with relatively sparse markers. When candidate regions are found, they are further investigated by association analysis. Because association studies require a dense set of markers, the cost of conventional genotyping can be very high. Here, we s ...
TRANSPOSON INSERTION SITE VERIFICATION
... Select the product size range 850-1000 from the select product size range field (Delete the other size ranges). Enter the following parameters: Primer size: 27 min 29 opt 31 max Primer tm: 60 min 65opt 70 max Primer GC content: 30 min 35 opt 40 max ...
... Select the product size range 850-1000 from the select product size range field (Delete the other size ranges). Enter the following parameters: Primer size: 27 min 29 opt 31 max Primer tm: 60 min 65opt 70 max Primer GC content: 30 min 35 opt 40 max ...
Lecture Notes
... IX) Molecular cytogenetics: Fluorescence in situ hybridization (FISH). A) Methodology 1) Metaphase chromosomes or interphase cells are fixed onto a microscope slide, the DNA is denatured and then hybridized with a probe that can be detected by fluorescence. The chromosomes or hybridized regions are ...
... IX) Molecular cytogenetics: Fluorescence in situ hybridization (FISH). A) Methodology 1) Metaphase chromosomes or interphase cells are fixed onto a microscope slide, the DNA is denatured and then hybridized with a probe that can be detected by fluorescence. The chromosomes or hybridized regions are ...
Lesson Plan, GeneChip® Microarrays: Teacher`s Guide
... may decide to use this module and the activities in it to supplement a unit on DNA, genetics, or even one on the Human Genome Project. The entire module is organized to go from a basic introduction of the GeneChip microarray and then build from there. After the basic introduction, the module moves o ...
... may decide to use this module and the activities in it to supplement a unit on DNA, genetics, or even one on the Human Genome Project. The entire module is organized to go from a basic introduction of the GeneChip microarray and then build from there. After the basic introduction, the module moves o ...
mRNA Expression Analysis
... Expression BeadChips are part of a complete gene expression solution that includes instrumentation, software, and reagent kits. Data analysis is straightforward, since known biologically relevant transcripts are annotated from heavily curated databases and probes are designed and validated by Illumi ...
... Expression BeadChips are part of a complete gene expression solution that includes instrumentation, software, and reagent kits. Data analysis is straightforward, since known biologically relevant transcripts are annotated from heavily curated databases and probes are designed and validated by Illumi ...
A Major Species of Mouse μ-opioid Receptor mRNA and Its
... Ample evidence has shown that 3⬘-UTR of mRNA plays an important role in gene regulation. The average length of 3⬘-UTR has increased dramatically throughout the evolutionary process, from 200 nucleotides in plant and fungi to 800 and more in humans and other vertebrates. This increase suggests a pote ...
... Ample evidence has shown that 3⬘-UTR of mRNA plays an important role in gene regulation. The average length of 3⬘-UTR has increased dramatically throughout the evolutionary process, from 200 nucleotides in plant and fungi to 800 and more in humans and other vertebrates. This increase suggests a pote ...
GenomeWeb Stanford Team Shows How Long Reads Can
... caused by variants that are difficult to identify with shorter reads, according to a recently published study. Researchers from Stanford University's Clinical Genomics Service reported last month in the pre-print server bioRxiv that they used Pacific Biosciences' Sequel instrument to sequence the wh ...
... caused by variants that are difficult to identify with shorter reads, according to a recently published study. Researchers from Stanford University's Clinical Genomics Service reported last month in the pre-print server bioRxiv that they used Pacific Biosciences' Sequel instrument to sequence the wh ...
Eurofins Legionella PCR (Polymerase Chain Reaction) Technical
... Advantages of Using the Legionella PCR Method Rather than the Culture Technique The traditional Legionella culture technique takes 10 days to arrive at a confirmed negative result as Legionella bacteria are slow growing in the laboratory. A further 3 days are required for confirmation in the case of ...
... Advantages of Using the Legionella PCR Method Rather than the Culture Technique The traditional Legionella culture technique takes 10 days to arrive at a confirmed negative result as Legionella bacteria are slow growing in the laboratory. A further 3 days are required for confirmation in the case of ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.