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Question_Bank_5th_Sem
Question_Bank_5th_Sem

... DNA, as genetic material/transforming principle was experimently proved by which of the following scientists? a. Friedrick Meischer and Griffith b. Watson and Crick c. Rosalin and Willkins d. Avery Mcleod and Mc Carty Double helical structure of dna was given by which of the following scientists a. ...
Dephosphorylation of Plasmid DNA Buffers and Solutions EDTA (0.5
Dephosphorylation of Plasmid DNA Buffers and Solutions EDTA (0.5

... electrophoresis through a 0.7% agarose gel containing ethidium bromide, using undigested plasmid DNA as a marker. If digestion is not complete, add more restriction enzyme and continue the incubation. 3. When digestion is complete, extract the sample once with phenol:chloroform and recover the DNA b ...
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Slide 1

... using the bases in a DNA molecule as a template • Translation – the creation of a protein using the bases in an RNA molecule as a template • RNA polymerase – an enzyme that adds RNA nucleotides to a new RNA molecule • Messenger RNA (mRNA) – an RNA molecule that carries instructions for the order of ...
AP BIOLOGY - Bremen High School District 228
AP BIOLOGY - Bremen High School District 228

... In an important experiment, a radioactively labeled bacteriophage was allowed to infect bacteria. In a first trial, the phage contained radioactive DNA, and radioactivity was detected inside the bacteria. Next, phage containing radioactive protein was used, and the radioactivity was not detected ins ...
DNA THE CODE OF LIFE 30 JANUARY 2013 Key Concepts
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... strands of DNA, forming two new polynucleotide chains. Hydrogen bonds form between the complementary base pairs. – Re-zips Each single strand of DNA becomes a new double strand. The two new double strands separate from each other as DNA replication is completed. Each new strand of DNA rewinds to for ...
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... 1. An RNA molecule usually consists of a single strand of nucleotides, not a double strand. This single-stranded structure is closely related to its function. 2. The sugar in RNA is ribose, rather than the deoxyribose sugar of DNA. 3. RNA contains the nitrogen base uracil (U) in place of thymine (T) ...
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DNA Replication – Lecture by Dr Mahmood S Choudhery

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Human Genetics

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Molecular Genetics
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... carried to the ribosomes by another type of RNA molecule, called transfer RNA (tRNA). A tRNA has two functional ends. One end picks up amino acids in the cytoplasm. 2. The other end is called the anticodon. It contains three nitrogen bases that can form a base pair with a matching codon in the mRNA. ...
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... travels to ribosome out in the cytoplasm tell the ribosome what protein to make. ...
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... consist of a phosphate group, simple sugar, and nitrogen base pairs. There are differences between the two molecules. RNA uses ribose as its sugar rather than the deoxyribose sugar in DNA. RNA is single stranded and the RNA molecule does not have thymine as a base. Uracil (U) replaces thymine (T) in ...
CH 12 Molecular Genetics (DNA, RNA, etc)
CH 12 Molecular Genetics (DNA, RNA, etc)

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Ch8 BacterialgeneticsPrt2HO.ppt
Ch8 BacterialgeneticsPrt2HO.ppt

... –  If not repaired, can lead to cell death; cancer in animals •  E.g., in humans, two breast cancer susceptibility genes code for DNA repair enzymes; mutations in either result in 80% probability of breast cancer ...
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... So, now, we know the nucleus controls the cell's activities through the chemical DNA, but how? It is the sequence of bases that determine which protein is to be made. The sequence is like a code that we can now interpret. The sequence determines which proteins are made and the proteins determine whi ...
DNA: Structure and Replication Deoxyribonucleic acid, or more
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... Deoxyribonucleic acid, or more simply DNA, is a complex molecule found in all living organisms. It is the chemical of which genes are composed. An understanding of the organization of this molecule has answered many questions. Scientists now know how chromosomes can duplicate during cell division an ...
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... RNA Synthesis - Most of the work of making RNA takes place during transcription. In transcription, segments of DNA serve as templates to produce complementary RNA molecules. In prokaryotes, RNA synthesis and protein synthesis takes place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s ...
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name date ______ period
name date ______ period

... a. changed proteins into DNA b. caused harmless bacteria to become deadly c. resulted in DNA molecules becoming proteins d. were designed to show the effect of heat on bacteria 31. Griffith’s experiments showed that a. dead bacteria could be brought back to life. B. harmful bacteria were hardier tha ...
DNA, RNA, and PROTEINS
DNA, RNA, and PROTEINS

... a. changed proteins into DNA b. caused harmless bacteria to become deadly c. resulted in DNA molecules becoming proteins d. were designed to show the effect of heat on bacteria 31. Griffith’s experiments showed that a. dead bacteria could be brought back to life. B. harmful bacteria were hardier tha ...
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Helicase



Helicases are a class of enzymes vital to all living organisms. Their main function is to unpackage an organism's genes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e., DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. There are many helicases resulting from the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases. The human genome codes for 95 non-redundant helicases: 64 RNA helicases and 31 DNA helicases. Many cellular processes, such as DNA replication, transcription, translation, recombination, DNA repair, and ribosome biogenesis involve the separation of nucleic acid strands that necessitates the use of helicases.
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