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Molecular Bio Questions1
Molecular Bio Questions1

... 7. How has the study of metagenomics allowed discovery of so many new species? How is it possible to know about these organisms without being able to grow them in a laboratory? ...
Slide 1
Slide 1

... Ex: Hold DNA out straight so that it has no Writhe, add of take out twist, then let fold up (Twist goes into Writhe). Normal DNA is negatively supercoiled, -0.06 = 6 turns for every 100 taken out. Why? Helps unwind DNA– makes it easier to uncoil, separate strands. Enzymes which do this called Topois ...
Slide 1
Slide 1

... • RNA polymerase separates the DNA strands and uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA • Laser Disk on transcription. ...
Biologically important reactions
Biologically important reactions

... Carbohydrates are commonly known as ______________________________. The monomer of carbohydrates are called ______________________________. The most important biological function of carbohydrates is the production of ____________. List the three elements found in carbohydrates. _____________________ ...
Harlem DNA Lab brochure
Harlem DNA Lab brochure

... winners. CSHL is ranked number one in the world by Thomson Reuters for the impact of its research in molecular biology and genetics. Harlem DNA Lab draws on the DNALC’s long experience in translating current biological research into hands-on learning activities and establishing teaching centers worl ...
Chargaff`s Rules - Rutgers University
Chargaff`s Rules - Rutgers University

... of bases in DNA (Marshal Nirenberg, Nobel Prize, Phys. & Med. 1968). Because human protein contains 20 amino acids, the DNA code must contain 20 code “words”, each representing a specific amino acid. The four DNA bases can be combined to a sufficient number of three DNA base groups, called codons, t ...
Protocol for End-It™ DNA End-Repair Kit
Protocol for End-It™ DNA End-Repair Kit

... of next-gen DNA sequencing adaptors. • Prepare double-stranded cDNA, produced from cellular RNA transcripts, for ligation of next-gen DNA sequencing adaptors. • Prepare sheared, nebulized, or restriction enzyme digested DNA for blunt-end ligation into plasmid, cosmid, fosmid, or BAC vectors. • Pr ...
i. dna structure and functions
i. dna structure and functions

... A. Amino acids do not have direct affinity for nucleic acids 1. Therefore, mRNA can not directly serve as a template for protein synthesis 2. There must exist an “adapter molecules” which can read the RNA sequence (codon) and bring with it the correct amino acids a) This is the function of tRNA mole ...
DNA - Duncanville ISD
DNA - Duncanville ISD

... RNA is a copy of DNA that goes out into the cytoplasm to tell the cell what to do in order to stay alive  RNA: ribonucleic acid  You can always make more RNA so it’s ok if it gets destroyed (You can’t make more DNA!!!) DNA RNA ...
e1. protein synthesis - Eric Hamber Secondary
e1. protein synthesis - Eric Hamber Secondary

... - Ribosomes move along messenger RNA reading codons and binding amino acids that are in the right place due to the transfer RNA (tRNA). - enzyme on ribosome catalyses the peptide bond - chain grows one amino acid at a time TERMINATION: - Ribosome reads “terminate” codon (UAG) and stops - releases pr ...
Real–time PCR identification and quantification of
Real–time PCR identification and quantification of

... different taxa or even across the eubacterial kingdom. Using various molecular analytic techniques small oligonucleotide probes or primers (16-20 nucleotides long) are able to detect the presence of complementary specific regions on the rRNA target molecule. With the modern high-end techniques used ...
DNA STRUCTURE
DNA STRUCTURE

... Non-Watson-Crick G:U base pairs represent additional regular base pairing in RNA, which enriched the capacity for self-complementarity. ...
DNA notes
DNA notes

...  a change in the DNA sequence  It’s a mistake that’s made during replication or transcription  can be harmful: diseases or deformities helpful: organism is better able to survive neutral: organism is unaffected  if a mutation occurs in a sperm or egg cell, that mutation is ...
12 1 DNA
12 1 DNA

...  In 1928, British scientist Fredrick Griffith was trying to learn how certain types of bacteria caused pneumonia.  He isolated two different strains of pneumonia bacteria from mice and grew them in his lab.  Performed the first major experiment that led to the discovery of DNA as the genetic mate ...
Mutations
Mutations

... Genetics history, 1920’s. Virulent cells have genes for making capsule which assists in infection. Mutant cells lack capsule, are harmless. Griffith combined heat killed, virulent cells with live, harmless mutants. The living cells took up the DNA from solution, changed into capsuleproducing, virule ...
Advanced Environmental Biotechnology II
Advanced Environmental Biotechnology II

... information on the structural diversity of environmental samples, or on the presence or absence of certain functional genes (e.g. genes ...
Biol-1406_Ch9Notes.ppt
Biol-1406_Ch9Notes.ppt

... • Each of two daughter cells gets an exact copy of parent cell’s genetic information • Duplication of the parent cell DNA is ...
Dna Deoxyribonucleic acid - Bethlehem Catholic High School
Dna Deoxyribonucleic acid - Bethlehem Catholic High School

... places along the chromosome. B. DNA replication is both semicontinuous and conservative. C. Multiple areas of replication occur along the chromosome at the same time. ...
Class 2
Class 2

... Non essential gene and α-complementation already present Large dsDNA region contains adenine methylation which should help limit the mismatch repair of the mutations arising during in vitro synthesis Easy to score large numbers of plaques Single stranded phage are readily sequenced ...
DNA – Deoxyribonucleic Acid
DNA – Deoxyribonucleic Acid

... They cut, splice together, & insert the modified DNA molecules from different species into bacteria or another type of cell that rapidly replicates and divides. The cells copy the foreign DNA right along with their own DNA. An example of this is the gene for human insulin inserted into a bacterium. ...
DNA - C. Shirley Science EJCHS
DNA - C. Shirley Science EJCHS

... They cut, splice together, & insert the modified DNA molecules from different species into bacteria or another type of cell that rapidly replicates and divides. The cells copy the foreign DNA right along with their own DNA. An example of this is the gene for human insulin inserted into a bacterium. ...
DNA replication
DNA replication

... The “parent” molecule has two complementary strands of DNA. Each is base paired by hydrogen bonding with its specific partner: A with T G with C ...
No Slide Title
No Slide Title

... higher accuracy than the Sanger chaintermination method ...
Review Sheet : DNA, RNA & Protein Synthesis
Review Sheet : DNA, RNA & Protein Synthesis

... It occurs during the S phase of cell cycle  b. Two complementary strands are duplicated.  c. The double strand unwinds and unzips while it is being duplicated.  d. The process is catalyzed by enzymes called DNA mutagens. ...
Chapter 12 Study Guide
Chapter 12 Study Guide

... 12 rules stating that in DNA a purine on one strand always pairs with a pyrimidine on the opposite strand 13 describes a microorganism or virus that causes disease and that is highly infectious 14 a Y-shaped point that results when the two strands of a DNA double helix separate so that the DNA molec ...
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DNA nanotechnology



DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.
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