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DNA replication
DNA replication

Structure of a six-finger transcription factor IIIA complex
Structure of a six-finger transcription factor IIIA complex

... Fingers 1–2–3 are separated by typical linker sequences, wrap smoothly around the major groove 2 Contacts are made with DNA bases mainly on the noncoding strand of the 5S rRNA gene. ...
8.2 Structure of DNA TEKS 3F, 6A, 6B
8.2 Structure of DNA TEKS 3F, 6A, 6B

... TEKS 3F, 6A, 6B ...
Nucleotides, nucleic acids and the genetic material
Nucleotides, nucleic acids and the genetic material

... • (deoxy-nucleotide-triphosphates) to hydrogen bond with their appropriate complementary dNTP on the single strand (A with T and G with C), and to form a covalent phosphodiester bond with the previous nucleotide of the same strand. The energy stored in the triphosphate is used to covalently bind eac ...
DNA replication
DNA replication

... • Each human cell contains 46 chromosomes (6 to 9 feet of DNA) • Your body contains 75-100 trillion of cells. • All of your DNA (when uncoiled and tied ...
Chapter 6 DNA Replication
Chapter 6 DNA Replication

... strand. This means that DNA polymerase cannot actually initiate synthesis of a DNA strand by joining the first nucleotides. Nucleotides must be added to the end of an already existing chain, called primer. The primer is not a DNA, but short stretch of RNA. Still another enzyme, primase, makes the pr ...
Nucleic Acids
Nucleic Acids

... 2- The DNA code is in Triplet Codons (short sequences of 3 nucleotides each) 3- Certain codons are translated by the cell into certain Amino acids. 4. Thus, the sequence of nucleotides in DNA indicate a sequence of Amino acids (Primary Structure) in a ...
Chapter 12 Study Guide Answer Key.notebook
Chapter 12 Study Guide Answer Key.notebook

... What enzyme unwinds and unzips the DNA into two separate strands? ...
DNA replication,mutation,repair
DNA replication,mutation,repair

... and further elongates the DNA chain while simultaneously removing the RNA primer with its 5’ to 3’ exonuclease activity ...
Name
Name

... discovered the DNA polymerase I, which is involved in the process of DNA repair. His son, Thomas Kornberg, later discovered DNA polymerase III, the main molecule responsible for DNA replication. ...
Human Genetics
Human Genetics

... The key to the constant width of the double helix is the specific pairing of purines and pyrimidines via hydrogen bonds The complementary base pairs are: - Adenine and guanine - Cytosine and thymine ...
ppt
ppt

... DNA in the process. ◦ Tools:  Restriction enzymes (Campbell – some one log into their student account please to show the class) – discovered in the 1960s by bacterial researchers ...
Objective Questions
Objective Questions

... 12) Transformation is the transfer of DNA from a donor to a recipient cell A) By a bacteriophage. B) As naked DNA in solution. C) By cell-to-cell contact. D) By crossing over. E) By sexual reproduction. 13) Genetic change in bacteria can be brought about by A) Mutation. B) Conjugation. C) Transducti ...
Biology 340 Molecular Biology
Biology 340 Molecular Biology

... Alu elements (human) ~300 bp element present at 1 million sites in the human genome (~10% of human DNA) Retroposons appear to have no functional significance. 4. Chromosome organization Bacterial chromosome: --circular DNA chromosome --naked DNA associated with + charged polyamines --DNA is tightly ...
Nucleic Acids - University of Idaho
Nucleic Acids - University of Idaho

... take a closer look at the nitrogenous bases that extend from each sugar molecule of the nucleotides in a nucleic acid. Slide 4 There are five different nitrogenous bases found in nucleic acids. These are cytosine, thymine, uracil, adenine and guanine. Due to their single ring structure, cytosine, th ...
Recap of 8.1 and 8.2
Recap of 8.1 and 8.2

... It passes from generation to generation without changing. 2. The two strands are linked only by hydrogen bonds: During DNA replication and protein synthesis, the strands can separate easily. 3. It’s a huge molecule: It can store vast amounts of information. 4. It’s coiled into a double helix. The in ...
PROTEIN SYNTHESIS
PROTEIN SYNTHESIS

... Steps of transcription cont… 4. RNA polymerase matches up complementary bases between DNA and RNA (A-U, C-G), using DNA as a template 5. RNA polymerase moves along the area of the DNA with the recipe, matching up complementary bases (elongation phase) 6. when it hits the “stop codon” mRNA drops off ...
The structure of a d5SICS-dNaM pairing - Digital USD
The structure of a d5SICS-dNaM pairing - Digital USD

... than one unnatural pair, using a technique called NMR spectroscopy. Thus far, NMR chemical shift assignments for this duplex have been largely completed which provide some information as to the structural configuration of the base pairs. Preliminary results indicate that one of the two adjacent 5SIC ...
Nucleic Acid • Nucleosides consist of a nitrogenous base and a
Nucleic Acid • Nucleosides consist of a nitrogenous base and a

Nucleic Acid • Nucleosides consist of a nitrogenous base and a
Nucleic Acid • Nucleosides consist of a nitrogenous base and a

... bases: o Adenine, guanine, cytosine, & thymine o Uracil is not found in DNA; it is found in RNA, which we will see later • Adenine and guanine are: o Double-ringed o Known as “purines” • Cytosine and thymine are: o Single-ringed o Known as “pyrimidines” • As a pneumonic, remember that “cytosine,” “t ...
Unit 4
Unit 4

... complements that along the other. Describe the structure of DNA, and explain what kind of chemical bond connects the nucleotides of each strand and what type of bond holds the two strands together.  DNA is a double helix. Purine and pyrimidine bases are stacked. There are ten layers of nitrogenous ...
Section 12–1 DNA (pages 287–294)
Section 12–1 DNA (pages 287–294)

... 22. Is the following sentence true or false? Adenine and guanine are larger molecules than true ...
Extracting DNA from Cells
Extracting DNA from Cells

... Now I have DNA for my genetic assay • Actually, I still have to remove the RNA (They purify together). I will use an enzyme to do this, RNAase, and then remove the enzyme, use ethanol to precipitate the DNA one more time, and resuspend it. • Then I will check the concentration of the DNA with a spe ...
Objective #2: Nucleic Acid Structure
Objective #2: Nucleic Acid Structure

... _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ ____________________________________ ...
LEQ: How do we splice new genes into DNA?
LEQ: How do we splice new genes into DNA?

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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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