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... DNA polymerases add on new nucleotides to the growing DNA strand – One nucleotide is added at a time to the 3’-OH group of the previous nucleotide – The 3’-OH group of the ribose sugar is covalently linked to the nucleoside triphosphate forming a phosphodiester ...
... DNA polymerases add on new nucleotides to the growing DNA strand – One nucleotide is added at a time to the 3’-OH group of the previous nucleotide – The 3’-OH group of the ribose sugar is covalently linked to the nucleoside triphosphate forming a phosphodiester ...
Summary of lesson
... Sample Answer: direction of replication, requires additional primers Both strands replicate with the same enzymes, but the lagging strand must replicate in short segments to make up for replicating away from the direction of the helicase. More on Okazaki fragments in the next segment. ...
... Sample Answer: direction of replication, requires additional primers Both strands replicate with the same enzymes, but the lagging strand must replicate in short segments to make up for replicating away from the direction of the helicase. More on Okazaki fragments in the next segment. ...
DNA REPLICATION
... • If you can wrap your head around the fact that an average cell cycle requires a full 20 minutes to complete – and the fact that each new cell that comes along requires a brand-spanking new copy of your DNA, you can imagine the legwork required to maintain the health of your body • Every time a cel ...
... • If you can wrap your head around the fact that an average cell cycle requires a full 20 minutes to complete – and the fact that each new cell that comes along requires a brand-spanking new copy of your DNA, you can imagine the legwork required to maintain the health of your body • Every time a cel ...
DNA ppt notes
... At the replication fork, DNA Polymerase III continuously adds complimentary nucleotides to exposed bases on 3’ end of new strand, this is called the leading strand DNA polymerase III must work away from the replication fork on the other strand, the lagging strand, to follow the 5’-3’ direction creat ...
... At the replication fork, DNA Polymerase III continuously adds complimentary nucleotides to exposed bases on 3’ end of new strand, this is called the leading strand DNA polymerase III must work away from the replication fork on the other strand, the lagging strand, to follow the 5’-3’ direction creat ...
DNA
... • Each side of DNA is called a “template mechanism” • Parent strand: original copy of DNA, used as template • Daughter strand: newly synthesized complement ...
... • Each side of DNA is called a “template mechanism” • Parent strand: original copy of DNA, used as template • Daughter strand: newly synthesized complement ...
Structure, replication and repair of DNA
... she had not been socially isolated) Chemical make up had been known for 30 years, but not the three-dimensional structure, which would give a clue as to how it worked. Nucleotides - sugar-phosphate backbone and nitrogenous bases. ...
... she had not been socially isolated) Chemical make up had been known for 30 years, but not the three-dimensional structure, which would give a clue as to how it worked. Nucleotides - sugar-phosphate backbone and nitrogenous bases. ...
Ch. 12.2: Replication of DNA
... Replication of DNA • Before a cell can divide by mitosis or meiosis, it must first make a copy of its chromosomes. • The DNA in the chromosomes is copied in a process called DNA replication. • Without DNA replication, new cells would have only half the DNA of their parents. • DNA is copied during in ...
... Replication of DNA • Before a cell can divide by mitosis or meiosis, it must first make a copy of its chromosomes. • The DNA in the chromosomes is copied in a process called DNA replication. • Without DNA replication, new cells would have only half the DNA of their parents. • DNA is copied during in ...
DNA sequencing - Winona State University
... The “half-steps” are called bases and they are adenine, guanine, thymine, and cytosine, which are abbreviated as A, G, T, and C. Chemically, A and G are purines, and C and T are pyrimidines. For a good fit, a pyrimidine must pair with a purine; in DNA, A bonds with T, and G bonds with C. These are w ...
... The “half-steps” are called bases and they are adenine, guanine, thymine, and cytosine, which are abbreviated as A, G, T, and C. Chemically, A and G are purines, and C and T are pyrimidines. For a good fit, a pyrimidine must pair with a purine; in DNA, A bonds with T, and G bonds with C. These are w ...
DNA polymerase
... Since DNA is antiparallel, synthesis occurs in opposite directions One strand in continuously synthesized leading strand (5’3’) The other is synthesized in short discontinuous strands - lagging strand (3’5’) Because of this DNA synthesis is called Semidiscontinuous ...
... Since DNA is antiparallel, synthesis occurs in opposite directions One strand in continuously synthesized leading strand (5’3’) The other is synthesized in short discontinuous strands - lagging strand (3’5’) Because of this DNA synthesis is called Semidiscontinuous ...
C16 DNA
... Replication fork – found at each end of a replication bubble, Yshaped region where new strands of DNA are elongating. DNA polyermases – catalyze elongation of new DNA and fixes mistakes made when DNA is copied. Free nucleotides serve as substrates for DNA polyermase. The nucleotides are nucleoside t ...
... Replication fork – found at each end of a replication bubble, Yshaped region where new strands of DNA are elongating. DNA polyermases – catalyze elongation of new DNA and fixes mistakes made when DNA is copied. Free nucleotides serve as substrates for DNA polyermase. The nucleotides are nucleoside t ...
DNA and RNA - Marist College, Athlone
... 15. Define the term DNA Profiling _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ ________________________________________________________ ...
... 15. Define the term DNA Profiling _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ ________________________________________________________ ...
DNA
... Replication: Entering the Forest • DNA Replication begins at special Sites called Origins of Replication • Where the two strands of DNA split is called replication bubbles (thousands) • Why do you need thousands of these bubbles? (Hint: The DNA molecule has 3 ...
... Replication: Entering the Forest • DNA Replication begins at special Sites called Origins of Replication • Where the two strands of DNA split is called replication bubbles (thousands) • Why do you need thousands of these bubbles? (Hint: The DNA molecule has 3 ...
4-Biochemical Properties of DNA and The Technology involve them
... is the result of an end to end fusion of two ancestral chromosomes. •As a result the two subtelomeric ends became the middle of chromosome 2, which is why we get hybridization of the probe there ...
... is the result of an end to end fusion of two ancestral chromosomes. •As a result the two subtelomeric ends became the middle of chromosome 2, which is why we get hybridization of the probe there ...
DNA - The Double Helix - OG
... function. The process of copying DNA is called replication. Replication in prokaryotes is different than in eukaryotes. Prokaryotes have circular DNA. In this process, replication starts at one point and proceeds in both directions until the entire circular chromosome has been replicated. Eukaryotic ...
... function. The process of copying DNA is called replication. Replication in prokaryotes is different than in eukaryotes. Prokaryotes have circular DNA. In this process, replication starts at one point and proceeds in both directions until the entire circular chromosome has been replicated. Eukaryotic ...
DNA-->RNA-->Proteins - Bakersfield College
... Each strand acts as template on which a new complementary strand is made by pairing the bases of new nucleotides with the bases of the template DNA strand New complementary strand is identical to old strand paired with template Have 2 identical double-stranded DNA molecules Each is half old DNA and ...
... Each strand acts as template on which a new complementary strand is made by pairing the bases of new nucleotides with the bases of the template DNA strand New complementary strand is identical to old strand paired with template Have 2 identical double-stranded DNA molecules Each is half old DNA and ...
REVIEW - TESADVBiology
... a. serve as a storage form for unused nucleotides. b. occupy space in the nucleus to keep the nucleus from collapsing. c. store information that tells the cells which proteins to make. d. serve as a template for making long, spiral carbohydrates. _____ 2. The two strands of a DNA molecule are held t ...
... a. serve as a storage form for unused nucleotides. b. occupy space in the nucleus to keep the nucleus from collapsing. c. store information that tells the cells which proteins to make. d. serve as a template for making long, spiral carbohydrates. _____ 2. The two strands of a DNA molecule are held t ...
DNA - California State University Channel Islands
... Introduction.-Studies of bacterial transformation and bacteriaphage infection'-‘ strongly indicate that deoxyribonucleic acid (DNA) can carry and transmit hereditary information and can direct its own replication. Hypotheses for the mechanism of DNA replication differ in the predictions they make co ...
... Introduction.-Studies of bacterial transformation and bacteriaphage infection'-‘ strongly indicate that deoxyribonucleic acid (DNA) can carry and transmit hereditary information and can direct its own replication. Hypotheses for the mechanism of DNA replication differ in the predictions they make co ...
Prepare for gel electrophoresis
... from your mother! Many studies use this DNA to determine how closely related different species of organisms are! ...
... from your mother! Many studies use this DNA to determine how closely related different species of organisms are! ...
DNA structure/genome/plasmid
... Recombinant plasmids made by molecular biologists have been designed to carry foreign DNA into bacterial ...
... Recombinant plasmids made by molecular biologists have been designed to carry foreign DNA into bacterial ...
MICROBIAL GENETICS
... 2- Chain initiation in which recognition protein called sigma factor (σ) attaches to RNA polymerase to put it at the correct site of the first nucleotide to be transcribed ...
... 2- Chain initiation in which recognition protein called sigma factor (σ) attaches to RNA polymerase to put it at the correct site of the first nucleotide to be transcribed ...
DNA replication
DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.