Construction of plant BAC libraries This document
... 12. On a clean Qtray cover on a workbench, reconstruct the gel by placing the unstained evennumbered pieces between their flanking odd-numbered stained pieces (FIGURE 2d). Extend the incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place th ...
... 12. On a clean Qtray cover on a workbench, reconstruct the gel by placing the unstained evennumbered pieces between their flanking odd-numbered stained pieces (FIGURE 2d). Extend the incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place th ...
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... of magnetic poles. Such modifications, however, often come at a price. Bigger beads, for example, generate greater drag and reduce, therefore, the temporal resolution of the experiment [24]. Likewise, the use of microfabricated devices [18] can produce forces in nN range due to a dramatic decrease i ...
... of magnetic poles. Such modifications, however, often come at a price. Bigger beads, for example, generate greater drag and reduce, therefore, the temporal resolution of the experiment [24]. Likewise, the use of microfabricated devices [18] can produce forces in nN range due to a dramatic decrease i ...
Deconstructing the Genome: DNA at High Resolution
... hemoglobins readily release their oxygen to cells, which have an even lower level of oxygen than any source of the gas. After birth, when oxygen is abundantly available in the lungs, adult hemoglobin, with its more relaxed kinetics of oxygen binding, allows for the most efficient pickup and delivery ...
... hemoglobins readily release their oxygen to cells, which have an even lower level of oxygen than any source of the gas. After birth, when oxygen is abundantly available in the lungs, adult hemoglobin, with its more relaxed kinetics of oxygen binding, allows for the most efficient pickup and delivery ...
This article was published in an Elsevier journal. The attached copy
... 10 min, the bioluminescent signals were quantified and recorded using an IVIS Imaging System (Xenogen) following the manufacturer’s instructions. The conditioned cell culture media (harvested before the luciferin addition) was returned to the same plate for further incubation. Measurements from the ...
... 10 min, the bioluminescent signals were quantified and recorded using an IVIS Imaging System (Xenogen) following the manufacturer’s instructions. The conditioned cell culture media (harvested before the luciferin addition) was returned to the same plate for further incubation. Measurements from the ...
Unit 4, Lesson 10 Chromosomes and Genetics
... Allow students about ten minutes to look at the website which explains the origin of the breed and what causes these goats to faint. If you do not have access to a computer lab, use computer projection to take the entire class through the site. IV. Genetic Disorders A. Usually caused by mutations – ...
... Allow students about ten minutes to look at the website which explains the origin of the breed and what causes these goats to faint. If you do not have access to a computer lab, use computer projection to take the entire class through the site. IV. Genetic Disorders A. Usually caused by mutations – ...
Transfer of genetic material between the chloroplast and nucleus
... pericentromeric regions play a significant role in facilitating the chloroplast–nuclear DNA flux. This equilibrium between integration and deletion is necessary to prevent the nuclear genome continuously expanding with the contribution of chloroplast-related sequences manifestly increasing. However, ...
... pericentromeric regions play a significant role in facilitating the chloroplast–nuclear DNA flux. This equilibrium between integration and deletion is necessary to prevent the nuclear genome continuously expanding with the contribution of chloroplast-related sequences manifestly increasing. However, ...
Chromosome anomalies course
... sequence, is imprinted and therefore silenced. AS is named after a British pediatrician, Dr. Harry Angelman, who first described the syndrome in 1965. People with AS are sometimes known as "angels", both because of the syndrome's name and because of their youthful, happy ...
... sequence, is imprinted and therefore silenced. AS is named after a British pediatrician, Dr. Harry Angelman, who first described the syndrome in 1965. People with AS are sometimes known as "angels", both because of the syndrome's name and because of their youthful, happy ...
Mosaic Isodicentric Y Chromosome in a Patient with Mixed Gonadal
... The phenotypic effect of idic(Y) is likely to depend on the presence or absence of important loci, such as the SRY and special genes that are responsible for spermatogenesis. The idic(Yp) chromosome has usually two short arms, which include SRY and Yq in variable sizes according to the breakpoints. ...
... The phenotypic effect of idic(Y) is likely to depend on the presence or absence of important loci, such as the SRY and special genes that are responsible for spermatogenesis. The idic(Yp) chromosome has usually two short arms, which include SRY and Yq in variable sizes according to the breakpoints. ...
Methods for detection of point mutations
... the analysis of DNA:DNA heteroduplices, but it may also be applied for the analysis of DNA:RNA heteroduplices [1]. When low amounts of mutant alleles are analyzed in a large background of wild-type DNA, sensitivity can be increased by separation and detection of fluorescencelabeled fragments on a DN ...
... the analysis of DNA:DNA heteroduplices, but it may also be applied for the analysis of DNA:RNA heteroduplices [1]. When low amounts of mutant alleles are analyzed in a large background of wild-type DNA, sensitivity can be increased by separation and detection of fluorescencelabeled fragments on a DN ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... the splinkerette PCR (spPCR) method for the isolation of flanking genomic DNA of any P-element or piggyBac. We report a simple and detailed protocol for spPCR. We use spPCR to 1) map a GAL4 enhancer trap located inside a natural transposon, pinpointing a master regulatory region for olfactory neuron ...
... the splinkerette PCR (spPCR) method for the isolation of flanking genomic DNA of any P-element or piggyBac. We report a simple and detailed protocol for spPCR. We use spPCR to 1) map a GAL4 enhancer trap located inside a natural transposon, pinpointing a master regulatory region for olfactory neuron ...
BIO450 Primer Design Tutorial
... Primers can also participate in intermolecular base pairing. This is base pairing between two different primer molecules. They may have the same sequence, or they may be the two different primers for the reaction, usually called the forward and reverse primers, the first being the complement to the ...
... Primers can also participate in intermolecular base pairing. This is base pairing between two different primer molecules. They may have the same sequence, or they may be the two different primers for the reaction, usually called the forward and reverse primers, the first being the complement to the ...
Incorporation of reporter molecule
... to 1 h at 72°C and terminated by adding 10 ml of stop solution [98% (v/v) formamide, 10 mM EDTA, 0.01% (w/v) bromophenol blue]. The unpuri®ed DNA samples were denatured (99°C for 5 min) and 3 ml aliquots loaded onto a denaturing 7 M urea±12% (v/v) polyacrylamide gel (Bio-Rad sequencing gel). After s ...
... to 1 h at 72°C and terminated by adding 10 ml of stop solution [98% (v/v) formamide, 10 mM EDTA, 0.01% (w/v) bromophenol blue]. The unpuri®ed DNA samples were denatured (99°C for 5 min) and 3 ml aliquots loaded onto a denaturing 7 M urea±12% (v/v) polyacrylamide gel (Bio-Rad sequencing gel). After s ...
Classification of nucleic acids structures by means of the
... branch includes those DNA secondary structures showing a positive CD band at 260 nm (Figure 1), whereas the left branch contains those structures which do not show this CD signature [10]. Now, we will study in detail each one of these two main branches. The left one contains in turn three clusters. ...
... branch includes those DNA secondary structures showing a positive CD band at 260 nm (Figure 1), whereas the left branch contains those structures which do not show this CD signature [10]. Now, we will study in detail each one of these two main branches. The left one contains in turn three clusters. ...
CapeTownGenomes
... Few genomes are completely sequenced. The completion and quality assurance needed for bacterial genomes is expensive, for larger eukaryotes even more so. ‘Finishing’ is the process by which a WGS shotgun assembly is completed (determine the sequence from any physical or sequence gaps) and furthe ...
... Few genomes are completely sequenced. The completion and quality assurance needed for bacterial genomes is expensive, for larger eukaryotes even more so. ‘Finishing’ is the process by which a WGS shotgun assembly is completed (determine the sequence from any physical or sequence gaps) and furthe ...
Bio 309F
... 29. Why have geneticists been able to identify several genes linked to the X chromosome in humans? A. the X chromosome is much easier to identify than the other chromosomes. B. the X chromosome is one of the smaller chromosomes, therefore easier to study C. only dominant genes are localized on the X ...
... 29. Why have geneticists been able to identify several genes linked to the X chromosome in humans? A. the X chromosome is much easier to identify than the other chromosomes. B. the X chromosome is one of the smaller chromosomes, therefore easier to study C. only dominant genes are localized on the X ...
introduction - Journal | Unair
... in men overall. The problem is that 70% of this infection in women and 50% in men are asymptomatic and can only be detected through accurate screening test. The nucleotide amplification using PCR technique could be used to amplify one DNA or RNA molecule of the microbe specific gene region that may ...
... in men overall. The problem is that 70% of this infection in women and 50% in men are asymptomatic and can only be detected through accurate screening test. The nucleotide amplification using PCR technique could be used to amplify one DNA or RNA molecule of the microbe specific gene region that may ...
Diagnostic protocol for
... of each slide. Controls of normal (pre-immune) serum at one dilution and of PBS are also added to the slide. Slides are enclosed in a humid chamber and incubated at room temperature for 30 min. The droplets are shaken off the slides and they are rinsed with PBS and then washed three times for 5 min ...
... of each slide. Controls of normal (pre-immune) serum at one dilution and of PBS are also added to the slide. Slides are enclosed in a humid chamber and incubated at room temperature for 30 min. The droplets are shaken off the slides and they are rinsed with PBS and then washed three times for 5 min ...
J Urol - prostata
... Univariate survival analyses were performed and Cox's proportional hazards model was used to identify significant prognostic factors. To assess how the experimental factors improve the capacity of the classical factors for predicting the patients who reach median survival, logistic regression multiv ...
... Univariate survival analyses were performed and Cox's proportional hazards model was used to identify significant prognostic factors. To assess how the experimental factors improve the capacity of the classical factors for predicting the patients who reach median survival, logistic regression multiv ...
Meiosis
... Drosophila, the pairing of homologous chromosomes occurs prior to meiosis. However, in many other organisms such as maize, oat, humans, and mice, homologous chromosomes are not associated with each other until zygotene. Regardless of when chromosomes pair, a major question in meiosis is, how do the ...
... Drosophila, the pairing of homologous chromosomes occurs prior to meiosis. However, in many other organisms such as maize, oat, humans, and mice, homologous chromosomes are not associated with each other until zygotene. Regardless of when chromosomes pair, a major question in meiosis is, how do the ...
Rolling circle transcription on smallest size double stranded DNA
... While DNA is primarily known as the information carrier of living organisms it has found recent application as a synthetic nano-scale engineering material. DNA has several key characteristics that makes the molecule highly suited for the creation of designed structures such as high stiffness, ease o ...
... While DNA is primarily known as the information carrier of living organisms it has found recent application as a synthetic nano-scale engineering material. DNA has several key characteristics that makes the molecule highly suited for the creation of designed structures such as high stiffness, ease o ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.