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enzymes - iLearning Centre
enzymes - iLearning Centre

... Highly specific that is each enzyme can only catalyse one kind of substrate Needed in small quantities because they are not used up but released at the end of a reaction Enzyme-catalyses reaction are reversible Can be slowed down or completely stopped by inhibitors. -e.g. : heavy metals such as lead ...
Enzymes - Food Science & Human Nutrition
Enzymes - Food Science & Human Nutrition



Enzymology
Enzymology

DNA Structure and Replication
DNA Structure and Replication

discovery of new enzymes in extreme environments through
discovery of new enzymes in extreme environments through

File
File

Nucleic Acid Notes (DNA,RNA) - Bremen High School District 228
Nucleic Acid Notes (DNA,RNA) - Bremen High School District 228

Essentiality and damage in metabolic networks
Essentiality and damage in metabolic networks

... number 2, 4, 6 and 10) are involved in the production of chorismate, which is an important link to the biosynthesis of aromatic aminoacids, folate and ubiquinone. The enzyme with the highest damage, ribose-phosphate-pyrophosphokinase, generates phosphoribosyl pyrophosphate, which is the initial comp ...
Control and Integration of Metabolism
Control and Integration of Metabolism

... substrate e.g. modulator molecule other than their substrate e.g. threonine dehydratase, the substrate is threonine and the modulator is L-isolecuien. • In Homotropic enzymes, the substrate also functions as the modulator. Homotropic enzymes contain two or more binding sites for the substrate- Modul ...
Enzyme Control of Metabolic Pathways
Enzyme Control of Metabolic Pathways

... Catabolic - in which larger molecules are broken down into smaller ones e.g. proteins into amino acids during digestion. ...
Chlamydia NAATs: update in the clinical and laboratory setting
Chlamydia NAATs: update in the clinical and laboratory setting

... Gen-Probe Aptima-Combo 2 Assay • Sensitivity 94 – 100%, specificity 98 – 100% • One sample, One test = Two results • CT/GC targets co-amplified and individually detected in a single tube • Suitable for large workloads • Transport medium allows storage up to 30C: 30 days for urine; 60 days for swab ...
Topic 5 Nucleic Acids as Drug Targets
Topic 5 Nucleic Acids as Drug Targets

... Double helix coils into a 3D shape - supercoiling ...
Enzyme Web Quest KEY
Enzyme Web Quest KEY

... 2. What do enzymes have to help them fit their substrates (the molecules that attach to the enzyme)? Enzymes have an active site to match up with their substrate. 3. What would happen without enzymes? Many important life processes would not happen without enzymes. True/False: Enzymes can help many d ...
Step 1
Step 1

... Step 6: Combine different motif scanning methods for optimal results -> sharp cutoff discriminates between positive and negative hits Scores of A. thaliana proteome scanning for GDS(L) motifs - Viterbi and posterior decoding combined ...
Enzymes
Enzymes

... per unit mass of protein (e.g. mmol/min/mg). If the specific activity is raised along purification then the protein preparation gets more pure. We have to consider that there are optimal conditions for E activity (pH, I, T, cofactor) and comparison is only valid if all values have been measured unde ...
Dinazyme C/S
Dinazyme C/S

Molecular Cell Biology
Molecular Cell Biology

... sharply bending the double helix → transcription ability ↑ Why is rich AT region ? ...
Nucleic Acid Deoxyribose Nucleic Acid (DNA)Ribose Nucleic Acid
Nucleic Acid Deoxyribose Nucleic Acid (DNA)Ribose Nucleic Acid

... •Double standed RNA can also exists and is generally similar to A-DNA (present is few viruses) ...
Enzyme Kinetics
Enzyme Kinetics

... 3. Hydrolase ...
DNA Technology20082009
DNA Technology20082009

... A Closer Look: Cutting and Pasting DNA with Restriction Enzymes – Recombinant DNA is produced by combining two ingredients: • A bacterial plasmid • The gene of interest ...
Position paper of the Working Group Food Biotechnology
Position paper of the Working Group Food Biotechnology

... The working group supports explicitly the safe and sustainable use of genetic engineering for the food industry. Previously quoted scientific concerns, for example as to remaining marker genes coding for antibiotics resistance have been overcome and invalidated by advanced protocols (construction of ...
Slide 1
Slide 1

Role of Deoxyribonucleic Acid Polymerase beta in Nuclear
Role of Deoxyribonucleic Acid Polymerase beta in Nuclear

... acid-soluble or else by lower amounts of DNAase followed by limited digestion by a 3’: 5’-exonuclease acting non-processively on duplex DNA (e.g. exonuclease I11 from Escherichia coli). This latter is sometimes termed ‘gapped’ DNA (Kornberg & Gefter, ...
Enzyme
Enzyme

... and experiments can then be carried out in which the lactose in milk is hydrolysed. Design of experiments to test the effect of temperature, pH and substrate concentration on the activity of enzymes. Experimental investigation of a factor affecting enzyme activity. (Practical 3) ...
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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.
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