Pourcel et al., Microbiology 2005
... Y. pseudotuberculosis represents an older, more diverged species from which Y. pestis probably emerged as a clone (Achtman et al., 1999). The three CRISPRs are present at least in some members of this species, and can be amplified using the Y. pestis primer pairs. We analysed the CRISPRs of nine str ...
... Y. pseudotuberculosis represents an older, more diverged species from which Y. pestis probably emerged as a clone (Achtman et al., 1999). The three CRISPRs are present at least in some members of this species, and can be amplified using the Y. pestis primer pairs. We analysed the CRISPRs of nine str ...
Regulation and Flexibility of Genomic Imprinting
... and maize (Zea mays) have shown a strong correlation between silent or active states with epigenetic marks, such as DNA methylation and histone modifications, but the nature of the primary imprint has not been clearly established for all imprinted genes. Phenotypes and expression patterns of imprint ...
... and maize (Zea mays) have shown a strong correlation between silent or active states with epigenetic marks, such as DNA methylation and histone modifications, but the nature of the primary imprint has not been clearly established for all imprinted genes. Phenotypes and expression patterns of imprint ...
Analysis of TALE superclass homeobox genes
... atypical homeodomain that had not been properly identified due to four separate frameshifts and other errors of the EST sequence within the homeobox. This gene was named ceh-25 and searches of the databases revealed several mammalian ESTs with high similarity, which were grouped together under a new ...
... atypical homeodomain that had not been properly identified due to four separate frameshifts and other errors of the EST sequence within the homeobox. This gene was named ceh-25 and searches of the databases revealed several mammalian ESTs with high similarity, which were grouped together under a new ...
HUMAN CHROMOSOMES
... dye or orcein are used for staining. This method provides information only about the number and morphology of chromosomes. The chromosomes could be grouped on the basis of their relative sizes and the relative lengths of their two arms, i.e. the positions of their centromeres. Chromosomal Banding If ...
... dye or orcein are used for staining. This method provides information only about the number and morphology of chromosomes. The chromosomes could be grouped on the basis of their relative sizes and the relative lengths of their two arms, i.e. the positions of their centromeres. Chromosomal Banding If ...
Egg production
... The poor reproductive efficiency of broiler breeders is caused initially by problems in the formation of the ovarian hierarchy and subsequently by poor persistency of egg laying and erratic production (1, 2, 3). The excessive and disorganised ovarian growth at the onset of lay has been a corollary o ...
... The poor reproductive efficiency of broiler breeders is caused initially by problems in the formation of the ovarian hierarchy and subsequently by poor persistency of egg laying and erratic production (1, 2, 3). The excessive and disorganised ovarian growth at the onset of lay has been a corollary o ...
A set reduction and pattern matching problem motivated by Allele
... the conserved regions (dark blue areas) of a family specific consensus sequence and used to amplify members of the gene family. 2) Analysis of the amplified family members identifies regions specific to two subsets of the family (shown in red). These regions allows the amplified regions to be divide ...
... the conserved regions (dark blue areas) of a family specific consensus sequence and used to amplify members of the gene family. 2) Analysis of the amplified family members identifies regions specific to two subsets of the family (shown in red). These regions allows the amplified regions to be divide ...
The aconitase of Escherichia cok purification of the
... described by Andrews et al. (1989). The dilutions of the primary antiserum and the biotinylated anti-rabbit antibody were 1 : 1000 and 1:500, respectively, while that of the streptavidin-biotinylated horseradish peroxidase complex was 1 : 3000. Analysis of aconitase activity in A-infected bacteria a ...
... described by Andrews et al. (1989). The dilutions of the primary antiserum and the biotinylated anti-rabbit antibody were 1 : 1000 and 1:500, respectively, while that of the streptavidin-biotinylated horseradish peroxidase complex was 1 : 3000. Analysis of aconitase activity in A-infected bacteria a ...
Maternal and paternal genomes contribute equally to the
... transcripts with parent-of-origin effects, for which the number that were maternally enriched approximately equalled the number that were paternally enriched. Because most transcripts were derived from the maternal and paternal genomes in near-equal amounts, we hypothesized that either equal amounts ...
... transcripts with parent-of-origin effects, for which the number that were maternally enriched approximately equalled the number that were paternally enriched. Because most transcripts were derived from the maternal and paternal genomes in near-equal amounts, we hypothesized that either equal amounts ...
Genome-wide analysis by SNP Array
... by FISH. There remain numerous syndromes linking ID, CA and dysmorphia of unknown origin which could be caused by chromosomal microrear-rangements that are not diagnosed by FISH. This represents a limitation of the FISH technology. FISH does not examine the whole genome and can therefore study only ...
... by FISH. There remain numerous syndromes linking ID, CA and dysmorphia of unknown origin which could be caused by chromosomal microrear-rangements that are not diagnosed by FISH. This represents a limitation of the FISH technology. FISH does not examine the whole genome and can therefore study only ...
Skeletal muscle actin mRNA. Characterization of the 3
... in one of the a amylase genes (31). I t can also be seen that the 5' half of plasmid pi06 insert is G-C rich while the 3' half of the insert is A-T rich. The insert contains many repeated and homologous sequences. The insert of plasmid pl50 overlaps parts of the inserts of both plasmids p749 and pl0 ...
... in one of the a amylase genes (31). I t can also be seen that the 5' half of plasmid pi06 insert is G-C rich while the 3' half of the insert is A-T rich. The insert contains many repeated and homologous sequences. The insert of plasmid pl50 overlaps parts of the inserts of both plasmids p749 and pl0 ...
chromosome3
... a) The short arms of two non-homologous chromosomes break and the two remaining large arms adhere b) The short arms are repeated elsewhere, so their absence is not usually a problem 2. People with this condition are called translocation carriers and have 45 chromosomes 3. Symptoms a) Range from mild ...
... a) The short arms of two non-homologous chromosomes break and the two remaining large arms adhere b) The short arms are repeated elsewhere, so their absence is not usually a problem 2. People with this condition are called translocation carriers and have 45 chromosomes 3. Symptoms a) Range from mild ...
Chpt8_RecombineDNA.doc
... mediated conjugal transfer of parts of chromosomes in E. coli (Chapter 1). Recombination between two phage during a mixed infection of bacteria is another example. Also, the retrieval system for post-replicative repair (Chapter 7) involves general recombination. The mechanism of recombination has be ...
... mediated conjugal transfer of parts of chromosomes in E. coli (Chapter 1). Recombination between two phage during a mixed infection of bacteria is another example. Also, the retrieval system for post-replicative repair (Chapter 7) involves general recombination. The mechanism of recombination has be ...
Primers BSHG06 - National Genetics Reference Laboratories
... We have developed a prototype specification for validated primer design and optimisation and have demonstrated its utility in the context of a fully automated sample processing system. We have also implemented an automated optimisation pipeline that will expedite the development of new primer set ...
... We have developed a prototype specification for validated primer design and optimisation and have demonstrated its utility in the context of a fully automated sample processing system. We have also implemented an automated optimisation pipeline that will expedite the development of new primer set ...
Unit 4, Lesson 10 Chromosomes and Genetics
... Ladies and gentlemen, do our chromosomes change over time? What causes them to change? A change in the structure of the chromosome is a genetic mutation. There are three ways chromosomes are generally mutated. All result in the organism to lose the ability to function normally. An example of a mutat ...
... Ladies and gentlemen, do our chromosomes change over time? What causes them to change? A change in the structure of the chromosome is a genetic mutation. There are three ways chromosomes are generally mutated. All result in the organism to lose the ability to function normally. An example of a mutat ...
Review over DNA, RNA, proteins, viruses, bacteria, DNA technology
... Essential knowledge 3.C.2: Biological systems have multiple processes that increase genetic variation. c. Sexual reproduction in eukaryotes involving gamete formation, including crossing-over during meiosis and the random assortment of chromosomes during meiosis, and fertilization serve to increase ...
... Essential knowledge 3.C.2: Biological systems have multiple processes that increase genetic variation. c. Sexual reproduction in eukaryotes involving gamete formation, including crossing-over during meiosis and the random assortment of chromosomes during meiosis, and fertilization serve to increase ...
ARTICLES - Weizmann Institute of Science
... Ji-Ping Z. Wang3 & Jonathan Widom2 Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the ...
... Ji-Ping Z. Wang3 & Jonathan Widom2 Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the ...
Genetic Control of X Chromosome Inactivation in Mice: Definition of
... or BALB females and CAST males. F1 progeny were then intercrossed to generate F2 females. In addition, we backcrossed B6CASTF1 females to B6 males or BALBCASTF1 females to BALB males for two to eight generations, selecting for the presence of CAST X chromosome alleles at each backcross generation. A ...
... or BALB females and CAST males. F1 progeny were then intercrossed to generate F2 females. In addition, we backcrossed B6CASTF1 females to B6 males or BALBCASTF1 females to BALB males for two to eight generations, selecting for the presence of CAST X chromosome alleles at each backcross generation. A ...
A Novel CpG Island Set Identifies Tissue-Specific
... CGIs are identified bioinformatically as DNA sequences with a base composition greater than 50% GþC and a CpG[o/ e] of more than 0.6 [23]. The DNA length over which this condition applies is critical. Initially the threshold most often used was 200 bp, whereas 500 bp is now more commonly applied [17] ...
... CGIs are identified bioinformatically as DNA sequences with a base composition greater than 50% GþC and a CpG[o/ e] of more than 0.6 [23]. The DNA length over which this condition applies is critical. Initially the threshold most often used was 200 bp, whereas 500 bp is now more commonly applied [17] ...
Elsevier Editorial System(tm) for Diagnostic Microbiology and
... (http://www.ncbi.nlm.nih.gov), the position 258 is usually occupied by a leucine (GOB-1, -3, -4, -5, -6, -12, -13) or isoleucine (GOB-8, -9, -10, -18) residues (Bellais et al., 2000). However, the blaGOB-like gene found in E. miricola was not cloned and GOB-like enzyme was not characterized because ...
... (http://www.ncbi.nlm.nih.gov), the position 258 is usually occupied by a leucine (GOB-1, -3, -4, -5, -6, -12, -13) or isoleucine (GOB-8, -9, -10, -18) residues (Bellais et al., 2000). However, the blaGOB-like gene found in E. miricola was not cloned and GOB-like enzyme was not characterized because ...
Gel Electrophoresis - Integrated DNA Technologies
... smaller than about 100 bases in an agarose gel because the sieving properties of agarose are not fine enough. On the other end of the scale, molecules longer than about 25,000 bp but shorter than around 2,000,000 bp will all run at the same rate. This is called limiting mobility. Nucleic acid molecu ...
... smaller than about 100 bases in an agarose gel because the sieving properties of agarose are not fine enough. On the other end of the scale, molecules longer than about 25,000 bp but shorter than around 2,000,000 bp will all run at the same rate. This is called limiting mobility. Nucleic acid molecu ...
Genetic recombination in plants
... maize, rice, tomato, soybean, wheat, and barley. In some cases, most notably Arabidopsis [l&15], tomato [16-191 and rice [ZO], these YAC and BAC clones are being organized into large contigs. These concigs represent a resource for investigating the relationship between the physical nature of chromos ...
... maize, rice, tomato, soybean, wheat, and barley. In some cases, most notably Arabidopsis [l&15], tomato [16-191 and rice [ZO], these YAC and BAC clones are being organized into large contigs. These concigs represent a resource for investigating the relationship between the physical nature of chromos ...
Nucleotide sequence and genome organization of foot-and
... Nucleic Acids Research tion (18). The sequence ot 7802 nonhomopolymeric nucleotides shown in Figure 3, represents the complete primary structure ot the L segment. Assuming additional 150 bases tor poly(C) and 400 tor the S segment this corresponds to 94 % of the FMDV genome indicating that the size ...
... Nucleic Acids Research tion (18). The sequence ot 7802 nonhomopolymeric nucleotides shown in Figure 3, represents the complete primary structure ot the L segment. Assuming additional 150 bases tor poly(C) and 400 tor the S segment this corresponds to 94 % of the FMDV genome indicating that the size ...
HPV DNA PARTITIONING DURING MITOSIS AS FOLLOWED
... Papillomaviruses are non-enveloped, icosahedral DNA tumor viruses with circular, double-stranded genomes of approximately 8 kb (Figure 2). Each PV is host speciesspecific with infectivity limited to either mucosal or cutaneous epithelium at specific body sites. As of 2010, more than 150 different HP ...
... Papillomaviruses are non-enveloped, icosahedral DNA tumor viruses with circular, double-stranded genomes of approximately 8 kb (Figure 2). Each PV is host speciesspecific with infectivity limited to either mucosal or cutaneous epithelium at specific body sites. As of 2010, more than 150 different HP ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.