Analysis of Genetic Toggle Switch Systems Encoded on Plasmids
... exclusive switch there is an overlap between the two promoter sites, so they cannot be occupied at the same time. The studies in Refs. [27] focused on the case in which the switch system is encoded on the chromosome, namely, each gene and its promoter site appears in a single copy. In addition, coop ...
... exclusive switch there is an overlap between the two promoter sites, so they cannot be occupied at the same time. The studies in Refs. [27] focused on the case in which the switch system is encoded on the chromosome, namely, each gene and its promoter site appears in a single copy. In addition, coop ...
gene mapping, marker gene mapping, marker-assisted
... The aquaculture genetics projects were developed based on long-term efforts of selective breeding programs. These projects are dependent upon one another so that the maximum benefits will be obtained by using the integrated genetic improvement approaches. For instance, gene mapping will produce more ...
... The aquaculture genetics projects were developed based on long-term efforts of selective breeding programs. These projects are dependent upon one another so that the maximum benefits will be obtained by using the integrated genetic improvement approaches. For instance, gene mapping will produce more ...
Ribotyping of Clostridium perfringens from industrially produced
... ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl. perfringens varied between 12 and 17. Typical ribotype patterns of Cl. perfringens were as follows: 7–11 bands between 23 kb and 4Æ3 kb, 0–4 bands between 4Æ3 kb and 2Æ0 kb and ...
... ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl. perfringens varied between 12 and 17. Typical ribotype patterns of Cl. perfringens were as follows: 7–11 bands between 23 kb and 4Æ3 kb, 0–4 bands between 4Æ3 kb and 2Æ0 kb and ...
29th International Conference on Animal Genetics ISAG2004/TOKYO
... 1) Creating the ultimate maps: Genomic sequencing of domesticated animals (P005) Lawrence B. Schook (Institute for Genomic Biology, University of Illinois, USA) ...
... 1) Creating the ultimate maps: Genomic sequencing of domesticated animals (P005) Lawrence B. Schook (Institute for Genomic Biology, University of Illinois, USA) ...
What Size Are Your Genes?
... The identification of specific mutations or gene rearrangements of certain genes are being developed as medical diagnostic tests to detect predisposition of disease states. Size determination of DNA fragments and genes are also essential for genetic engineering and DNA technology-based experiments. Ad ...
... The identification of specific mutations or gene rearrangements of certain genes are being developed as medical diagnostic tests to detect predisposition of disease states. Size determination of DNA fragments and genes are also essential for genetic engineering and DNA technology-based experiments. Ad ...
Rates of Spontaneous Mutation
... lytic RNA viruses, a retrovirus or retrotransposon chromosome replicates precisely three times per infective cycle. Transcription by the host RNA polymerase produces an RNA genome. Reverse transcriptase then catalyzes two replications to generate a DNA-based chromosome that integrates into the host ...
... lytic RNA viruses, a retrovirus or retrotransposon chromosome replicates precisely three times per infective cycle. Transcription by the host RNA polymerase produces an RNA genome. Reverse transcriptase then catalyzes two replications to generate a DNA-based chromosome that integrates into the host ...
Updated slides on graph algorithms for DNA sequencing
... • Practicality: SBH is still impractical. As DNA microarray technology improves, SBH may become practical in the future • Practicality again: Although SBH is still impractical, it spearheaded expression analysis and SNP analysis techniques ...
... • Practicality: SBH is still impractical. As DNA microarray technology improves, SBH may become practical in the future • Practicality again: Although SBH is still impractical, it spearheaded expression analysis and SNP analysis techniques ...
Clin Infect Dis. - Repositorio Académico UPC
... 0.5 U of Phusion polymerase (Finnzyme OY) in high-fidelity Phusion buffer, with a final concentration of 350 lM of each deoxyribonucleotide triphosphates and 4 mM MgCl2. The primers used for the detection of the eaeA gene were forward 5#-ATGCTTAGTGCTGGTTTAGG-3# and reverse 5#GCCTTCATCATTTCGCTTTC-3# ...
... 0.5 U of Phusion polymerase (Finnzyme OY) in high-fidelity Phusion buffer, with a final concentration of 350 lM of each deoxyribonucleotide triphosphates and 4 mM MgCl2. The primers used for the detection of the eaeA gene were forward 5#-ATGCTTAGTGCTGGTTTAGG-3# and reverse 5#GCCTTCATCATTTCGCTTTC-3# ...
A Novel Mouse Chromosome 17 Hybrid Sterility Locus
... X-derived region and a distal Yderived region. For example, t t, D denotes a proximal partial t haplotype that originated within a M . domesticus mouse, D c-, t denotes a distal partial t haplotype, and S c* D and D c-, S denote structurally reciprocal forms of recombinant M. spretus-M. domesticus c ...
... X-derived region and a distal Yderived region. For example, t t, D denotes a proximal partial t haplotype that originated within a M . domesticus mouse, D c-, t denotes a distal partial t haplotype, and S c* D and D c-, S denote structurally reciprocal forms of recombinant M. spretus-M. domesticus c ...
Systematic and Applied Microbiology - digital
... Results and discussion These sequences of strains BIFI-56 and BIFI-58 showed a 100% identity to the same fragment of the16S rRNA sequence from the E. faecium type strain (ATCC 19434, DSM 20477).Therefore the 16S rRNA sequence revealed that both strains were E. faecium isolates. In order to know if ...
... Results and discussion These sequences of strains BIFI-56 and BIFI-58 showed a 100% identity to the same fragment of the16S rRNA sequence from the E. faecium type strain (ATCC 19434, DSM 20477).Therefore the 16S rRNA sequence revealed that both strains were E. faecium isolates. In order to know if ...
Telomereled bouquet formation facilitates homologous chromosome
... and several cosmid DNAs as probes for unique sequences on chromosome II (see Figure 1). Three other probes were also used: YIp10.4 for rRNA gene clusters (rDNA) located at both termini of chromosome III, cos212 for telomeres of chromosomes I and II, and pRS140 for the centromeres of all chromosomes. ...
... and several cosmid DNAs as probes for unique sequences on chromosome II (see Figure 1). Three other probes were also used: YIp10.4 for rRNA gene clusters (rDNA) located at both termini of chromosome III, cos212 for telomeres of chromosomes I and II, and pRS140 for the centromeres of all chromosomes. ...
A global picture of tRNA genes in plant genomes
... For each of the six genomes included in our survey, we determined the number of tDNAs and tRNA pseudogenes as well as the number of organellar tRNA genes inserted into the nuclear genomes (Table 1). We cannot exclude the possibility that a few of the transferred organellar tRNA genes became function ...
... For each of the six genomes included in our survey, we determined the number of tDNAs and tRNA pseudogenes as well as the number of organellar tRNA genes inserted into the nuclear genomes (Table 1). We cannot exclude the possibility that a few of the transferred organellar tRNA genes became function ...
Report Organelles in Blastocystis that Blur the
... same organelles is more suggestive of hydrogenosomal metabolism. To clarify the metabolic properties of the Blastocystis MLOs, we used 3330 clusters constructed from our EST data for a comparative BLAST search against the yeast and human mitochondrial proteomes [14, 15]. In addition, the KEGG Automa ...
... same organelles is more suggestive of hydrogenosomal metabolism. To clarify the metabolic properties of the Blastocystis MLOs, we used 3330 clusters constructed from our EST data for a comparative BLAST search against the yeast and human mitochondrial proteomes [14, 15]. In addition, the KEGG Automa ...
Journal of Microbiology and Biotechnology
... Construction and Cloning of ipdC Candidates in pUC19 Vector ...
... Construction and Cloning of ipdC Candidates in pUC19 Vector ...
RELIC – A bioinformatics server for combinatorial
... selected peptides; serially performs this function for multiple protein sequences using the same peptide sequence set, then compiles a list of those proteins so that they are ranked by similarity factor running from most similar to least similar. This program is capable of sorting an entire genome-d ...
... selected peptides; serially performs this function for multiple protein sequences using the same peptide sequence set, then compiles a list of those proteins so that they are ranked by similarity factor running from most similar to least similar. This program is capable of sorting an entire genome-d ...
Missouri Western State University
... first and second, second and third, all three. • The following slide shows Northern Hemisphere of the 2-spatula burnt pancake graph on a globe. ...
... first and second, second and third, all three. • The following slide shows Northern Hemisphere of the 2-spatula burnt pancake graph on a globe. ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.