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The lower Vmax is consistent with the mutation resulting in a
The lower Vmax is consistent with the mutation resulting in a

... 10. The plasma membrane of human cells contains a ubiquitously distributed family of tissue-specific receptor complexes involved in regulating cell adhesion, growth, and differentiation. These complexes contain a member of the tetraspanin superfamily of transmembrane proteins that is non-covalently ...
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... 10. The plasma membrane of human cells contains a ubiquitously distributed family of tissue-specific receptor complexes involved in regulating cell adhesion, growth, and differentiation. These complexes contain a member of the tetraspanin superfamily of transmembrane proteins that is non-covalently ...
RESEARCH NOTES
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... Wild type Neuro~oro crow 74-OR23-1A (FGSC”987) was grown on Vogel’s medium N with 2% ogor ot 25’C for 5 days. The conidio were harvested o&T&red to remove myceliol frogments. An oliquot of the resulting suspension was dried ot 55’C and the volume of the suspension was adjusted to obtain a concentrat ...
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Coenzymes and Cofactors (PDF Available)

... serine hydroxyl is to form, via nucleophilic attack, an acyl enzyme intermediate that is more readily hydrolysed than the substrate amide bond. Note also the role of aspartate and histidine as general acid–base catalysts in this mechanism: (1) they serve to deprotonate the serine, making it more nuc ...
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... Enzyme Catalysis • Speed of the enzyme action depends on the concentration of the enzyme and substrate. – As enzyme concentration increases, enzyme activity will increase to a point. – As substrate concentration increases, enzyme activity will increase to a point. ...
Lecture notes Chapter 22-23
Lecture notes Chapter 22-23

< 1 ... 73 74 75 76 77 78 79 80 81 ... 126 >

Catalytic triad



A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.
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