Amino Acids - Chavis Biology
... 2. On the general structure of an amino acid shown above, circle and label the carboxyl group. The carboxyl group makes the molecule behave like a weak acid (hence “amino acid”). 3. On the general structure of an amino acid shown above, circle and label the amine group (hence “amino acid”). 4. What ...
... 2. On the general structure of an amino acid shown above, circle and label the carboxyl group. The carboxyl group makes the molecule behave like a weak acid (hence “amino acid”). 3. On the general structure of an amino acid shown above, circle and label the amine group (hence “amino acid”). 4. What ...
Amino Acids - Chavis Biology
... 2. On the general structure of an amino acid shown above, circle and label the carboxyl group. The carboxyl group makes the molecule behave like a weak acid (hence “amino acid”). 3. On the general structure of an amino acid shown above, circle and label the amine group (hence “amino acid”). 4. What ...
... 2. On the general structure of an amino acid shown above, circle and label the carboxyl group. The carboxyl group makes the molecule behave like a weak acid (hence “amino acid”). 3. On the general structure of an amino acid shown above, circle and label the amine group (hence “amino acid”). 4. What ...
II. The Steps of Translation
... Each kind of tRNA has a sequence of 3 unpaired nucleotides — the anticodon — which can bind, following the rules of base pairing, to the complementary triplet of nucleotides — the codon — in a messenger RNA (mRNA) molecule. Just as DNA replication and transcription involve base pairing of nucleotide ...
... Each kind of tRNA has a sequence of 3 unpaired nucleotides — the anticodon — which can bind, following the rules of base pairing, to the complementary triplet of nucleotides — the codon — in a messenger RNA (mRNA) molecule. Just as DNA replication and transcription involve base pairing of nucleotide ...
Activities for the -Helix and -Sheet Construction Kit
... The primary sequence of a protein, composed of amino acids, determines the organization of the sequence into the secondary structure. There are two periodic secondary structure motifs, α-helix and β-sheet. Proteins can be composed of primarily α-helices (for example, β-globin) or β-sheets (for examp ...
... The primary sequence of a protein, composed of amino acids, determines the organization of the sequence into the secondary structure. There are two periodic secondary structure motifs, α-helix and β-sheet. Proteins can be composed of primarily α-helices (for example, β-globin) or β-sheets (for examp ...
MICROBIAL PHYSIOLOGY AND BIOCHEMISTRY
... ranges varying from mM to M values. These weak interactions are quite similar to those that are involved in protein folding, i.e. electrostatic and hydrogen bonding, hydrophobic interactions and van der Waal forces. Note that van der Waal forces are significant only when multiple substrate atoms com ...
... ranges varying from mM to M values. These weak interactions are quite similar to those that are involved in protein folding, i.e. electrostatic and hydrogen bonding, hydrophobic interactions and van der Waal forces. Note that van der Waal forces are significant only when multiple substrate atoms com ...
Book Problems Chapter 2
... The transporter must include a cytosolic nucleotide binding site that changes its conformation when its bound ATP is hydrolyzed to ADP. This conformational change must be communicated to the membrane-spanning portion of the protein, where the transported substrate binds. (b) Overexpression of an MDR ...
... The transporter must include a cytosolic nucleotide binding site that changes its conformation when its bound ATP is hydrolyzed to ADP. This conformational change must be communicated to the membrane-spanning portion of the protein, where the transported substrate binds. (b) Overexpression of an MDR ...
IDENTIFICATION OF LEAD COMPOUNDS WITH COBRA VENOM NEUTRALISING ACTIVITY IN
... haemolytic, haemorrhagic, platelet aggregation and oedema inducing activity [12]. In Uniport database HIS47 and ASP90 were marked as active residue of this enzyme. HIS47 is the preceding residue of ASP48 in which Ca2+ binds during catalytic reaction, hence, it was taken as active residue for docking ...
... haemolytic, haemorrhagic, platelet aggregation and oedema inducing activity [12]. In Uniport database HIS47 and ASP90 were marked as active residue of this enzyme. HIS47 is the preceding residue of ASP48 in which Ca2+ binds during catalytic reaction, hence, it was taken as active residue for docking ...
Amino Acid Metabolism (day-2)
... How Do Organisms Synthesize Amino Acids? • Plants and microorganisms can make all 20 amino acids and all other needed N metabolites • In these organisms, glutamate is the source of N, via transamination (aminotransferase) reactions of α-keto acid analogue of the amino acid • Mammals can make only 1 ...
... How Do Organisms Synthesize Amino Acids? • Plants and microorganisms can make all 20 amino acids and all other needed N metabolites • In these organisms, glutamate is the source of N, via transamination (aminotransferase) reactions of α-keto acid analogue of the amino acid • Mammals can make only 1 ...
ENZYMES
... – Lipase works only on __________ – Sucrase works only on ____________ – Protease works only on ____________ – __________ works only on fructose ...
... – Lipase works only on __________ – Sucrase works only on ____________ – Protease works only on ____________ – __________ works only on fructose ...
DNA polymerase active site is highly mutable
... MgCl2, 0.4 mg of activated calf thymus DNA, 10 M each dNTP, 0.25 mCi of [␣-32P]dATP, and 1 l of WT or mutant Taq pol. Incubations were at 72°C for 5 min, and reactions were stopped with the addition of 100 l of 0.1 M sodium pyrophosphate, followed by 0.5 ml of 10% trichloroacetic acid (TCA). Poly ...
... MgCl2, 0.4 mg of activated calf thymus DNA, 10 M each dNTP, 0.25 mCi of [␣-32P]dATP, and 1 l of WT or mutant Taq pol. Incubations were at 72°C for 5 min, and reactions were stopped with the addition of 100 l of 0.1 M sodium pyrophosphate, followed by 0.5 ml of 10% trichloroacetic acid (TCA). Poly ...
Structural and functional studies on C4b
... thawing of native C4 or by treatment of the native C4 with 50 mM methylamine at 37~ for 1 h. The complete inactivation of the purified C4 was demonstrated by the cleavage of the C4i c~i chain by factor I in the presence of C4BP and the fact that the C4i was insensitive to Cls. The C4 haemolytic assa ...
... thawing of native C4 or by treatment of the native C4 with 50 mM methylamine at 37~ for 1 h. The complete inactivation of the purified C4 was demonstrated by the cleavage of the C4i c~i chain by factor I in the presence of C4BP and the fact that the C4i was insensitive to Cls. The C4 haemolytic assa ...
Vitamins and Coenzymes - KSU - Home
... Structure of β-Carbonic Anhydrase • Found in plans which is an evolutionarily distinct enzyme but participates in the same reaction and also uses a Zn2+ in its active site • It helps raise the concentration of CO2 within the chloroplast to increase the carboxylation rate of the enzyme Rubisco • It ...
... Structure of β-Carbonic Anhydrase • Found in plans which is an evolutionarily distinct enzyme but participates in the same reaction and also uses a Zn2+ in its active site • It helps raise the concentration of CO2 within the chloroplast to increase the carboxylation rate of the enzyme Rubisco • It ...
CITRIC ACID CYCLE
... substrates from one catalytic site to another ‒ substrate channeling minimizes side reactions ‒ regulation of activity of one subunit affects the entire complex ...
... substrates from one catalytic site to another ‒ substrate channeling minimizes side reactions ‒ regulation of activity of one subunit affects the entire complex ...
Urinary Amino Acids Profile of Vegetarians and Non
... Amino acids are the primary components of proteins and they are essential to life (Elliot and Elliot, 2001). Humans ingest far more protein (amino acids) than they need for replacement of endogenous proteins. These excess amino acids cannot be stored and thus are catabolised. The ultimate fate of th ...
... Amino acids are the primary components of proteins and they are essential to life (Elliot and Elliot, 2001). Humans ingest far more protein (amino acids) than they need for replacement of endogenous proteins. These excess amino acids cannot be stored and thus are catabolised. The ultimate fate of th ...
Enzyme kinetics and its relevance to enzyme assay
... about 90% of patients in the early stages of jaundice due to virus hepatitis the serum alanine aminotransferase value exceeded that of the aspartate aminotransferase whereas at Westminster Hospital probably less than 10% showed such a relationship. The difference proved to be due to the use in the P ...
... about 90% of patients in the early stages of jaundice due to virus hepatitis the serum alanine aminotransferase value exceeded that of the aspartate aminotransferase whereas at Westminster Hospital probably less than 10% showed such a relationship. The difference proved to be due to the use in the P ...
Catalytic triad
A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.