Purification and characterization of pyruvate decarboxylase from
... carried out at pH 6.5, as lower pH values resulted in less enzyme adsorption and recovery from the column, and cation exchange was unsuccessful. The presence of the cofactors TPP and MgS04 was essential for enzyme stability. The enzyme was unstable at pH values above 7.5 ; instability of pyruvate de ...
... carried out at pH 6.5, as lower pH values resulted in less enzyme adsorption and recovery from the column, and cation exchange was unsuccessful. The presence of the cofactors TPP and MgS04 was essential for enzyme stability. The enzyme was unstable at pH values above 7.5 ; instability of pyruvate de ...
Hormone sensitive lipase: structure, function and
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
Glutamate Dehydrogenases: Enzymology, Physiological
... (Fig. 1). Because of the reaction it catalyses, the main role of GDH is glutamate catabolism and ammonium assimilation. However, other physiological roles for GDH have been described in some organisms, as we will see below. ...
... (Fig. 1). Because of the reaction it catalyses, the main role of GDH is glutamate catabolism and ammonium assimilation. However, other physiological roles for GDH have been described in some organisms, as we will see below. ...
aminoacids
... It is often found in the active site of enzymes Imidazole ring can act as electron acceptor/donor in an enzyme catalyzed reactions Aminoacids ...
... It is often found in the active site of enzymes Imidazole ring can act as electron acceptor/donor in an enzyme catalyzed reactions Aminoacids ...
Catalytic Mechanism and Regulation of Mammalian Adenylyl Cyclases
... The main stimulatory effect of adenylyl cyclase by hormones and neurotransmitters is mediated by Gsa, the a subunit of Gs protein that stimulates adenylyl cyclase. Although each of the nine isoforms (types I–IX) of mammalian adenylyl cyclases has its unique and diverse regulation (Table 1), they sha ...
... The main stimulatory effect of adenylyl cyclase by hormones and neurotransmitters is mediated by Gsa, the a subunit of Gs protein that stimulates adenylyl cyclase. Although each of the nine isoforms (types I–IX) of mammalian adenylyl cyclases has its unique and diverse regulation (Table 1), they sha ...
Liining
... ever, show an antigenic activity which is suf?cient to evaporation the residue is puri?ed by gel ?ltration on enable the polypeptide to react with corresponding Sephadex G 25 Fine in 0.1 M NH4HCO3. After lyophili zation of the peptide a satisfactory amino acid analysis is 40 antibodies. Moreover, th ...
... ever, show an antigenic activity which is suf?cient to evaporation the residue is puri?ed by gel ?ltration on enable the polypeptide to react with corresponding Sephadex G 25 Fine in 0.1 M NH4HCO3. After lyophili zation of the peptide a satisfactory amino acid analysis is 40 antibodies. Moreover, th ...
Modification of the Enzymatic Activity of Renin by
... These samples were then adjusted to pH 7.4 by addition of 1 N NaOH and were again incubated for 18 hours at 4°C. To determine if acidification denatures a renin inhibitor in plasma, untreated plasma and plasma acidified both by dialysis and by addition of HC1 were added to renin-renin substrate. A p ...
... These samples were then adjusted to pH 7.4 by addition of 1 N NaOH and were again incubated for 18 hours at 4°C. To determine if acidification denatures a renin inhibitor in plasma, untreated plasma and plasma acidified both by dialysis and by addition of HC1 were added to renin-renin substrate. A p ...
The activity and kinetic properties of cellulases in substrates
... on cellulase, even as both of them are alkaline earth metals. From the data presented in Figure 3, the kinetic character of Mg2+ was a competitive inhibitor of cellulase. Endoglucanases had two conserved glutamate residues at the active site [8]. Metal ions might bind to these residual radicals to i ...
... on cellulase, even as both of them are alkaline earth metals. From the data presented in Figure 3, the kinetic character of Mg2+ was a competitive inhibitor of cellulase. Endoglucanases had two conserved glutamate residues at the active site [8]. Metal ions might bind to these residual radicals to i ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... (>50% inactive clones) produced no mutants with increased activity or stability against H2O2 and elevated temperature (3,300 colonies screened). Screening lower error-rate libraries produced several mutants with higher total activity (1.45–1.8-fold). Two of those (HRP H1-8H10, HRP H16E1) also retain ...
... (>50% inactive clones) produced no mutants with increased activity or stability against H2O2 and elevated temperature (3,300 colonies screened). Screening lower error-rate libraries produced several mutants with higher total activity (1.45–1.8-fold). Two of those (HRP H1-8H10, HRP H16E1) also retain ...
The Amino-acid Sequence in the Phenylalanyl Chain of Insulin
... slow-moving peptides it was necessary to run the solvent considerably farther than the end of the filter paper. This was accomplished by attaching a second folded sheet of filter paper to the front of the chromatogram. Where this was done the number of lengths of filter paper through which the solve ...
... slow-moving peptides it was necessary to run the solvent considerably farther than the end of the filter paper. This was accomplished by attaching a second folded sheet of filter paper to the front of the chromatogram. Where this was done the number of lengths of filter paper through which the solve ...
Contents - Elsevier
... mouse,6 and for bacterial lactate dehydrogenases.6–8 In all of these the polypeptide is folded nearly identically. The structures of the homologous cytosolic and mitochondrial isoenzymes of malate dehydrogenase are also similar,9–11 as are those of the bacterial enzyme.12,13 All of these proteins co ...
... mouse,6 and for bacterial lactate dehydrogenases.6–8 In all of these the polypeptide is folded nearly identically. The structures of the homologous cytosolic and mitochondrial isoenzymes of malate dehydrogenase are also similar,9–11 as are those of the bacterial enzyme.12,13 All of these proteins co ...
![Metabolism of [14C]glutamate and [14C]glutamine by](http://s1.studyres.com/store/data/002383503_1-555972945f32c24447774c3be1b0f14a-300x300.png)
Metabolism of [14C]glutamate and [14C]glutamine by
... To examine pathways of glutamate and glutamine metabolism in the ectomycorrhizal fungus Paxillus involutus, tracer kinetic experiments were performed using ~-[U-l~C]glutamate and ~-[U-~~C]glutamine and the enzyme inhibitors methionine sulfoximine (MSX), azaserine (AZA) and aminooxyacetate (AOA). Whe ...
... To examine pathways of glutamate and glutamine metabolism in the ectomycorrhizal fungus Paxillus involutus, tracer kinetic experiments were performed using ~-[U-l~C]glutamate and ~-[U-~~C]glutamine and the enzyme inhibitors methionine sulfoximine (MSX), azaserine (AZA) and aminooxyacetate (AOA). Whe ...
PowerPoint Presentation - Biotin Conclusion and Discussion
... – often found bound to protein • biocytin o or biotinyllysine ...
... – often found bound to protein • biocytin o or biotinyllysine ...
Mammalian CSAD and GADL1 have distinct biochemical properties
... biosynthesis of taurine. In the present study, we compared the catalytic properties, inhibitor sensitivity and expression profiles of GADL1 and CSAD in brain tissue. In mouse and human brain we observed distinct patterns of expression of the PLP-dependent decarboxylases CSAD, GADL1 and glutamic acid ...
... biosynthesis of taurine. In the present study, we compared the catalytic properties, inhibitor sensitivity and expression profiles of GADL1 and CSAD in brain tissue. In mouse and human brain we observed distinct patterns of expression of the PLP-dependent decarboxylases CSAD, GADL1 and glutamic acid ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
The Chemistry and Evolution of Enzyme Function
... member of such great academic institutions. Friends mean freedom. To Anna, Bruno and Claire for sharing my passion for cooking and being an unlimited source of social gatherings and walks alongside Paradise. To Matthias, Ulla and Nidhi for your everlasting bad jokes (see beginning and end of Acknowl ...
... member of such great academic institutions. Friends mean freedom. To Anna, Bruno and Claire for sharing my passion for cooking and being an unlimited source of social gatherings and walks alongside Paradise. To Matthias, Ulla and Nidhi for your everlasting bad jokes (see beginning and end of Acknowl ...
Catalytic triad
A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.